根管消毒对初级内牙感染中存在的细菌体的影响:下一代测序研究
Theeb Abdullah Alquria1, Aneesha Acharya2,3, Patricia Tordik1
1Department of Advanced Oral Sciences and Therapeutics, School of Dentistry, University of Maryland, Baltimore, Maryland, USA.
International endodontic journal
|May 3, 2024
概括
化学机械制剂 (CMP) 含有2.5%的二酸盐显著降低了细菌负载,并改变了牙中的微生物群体,主要是内牙感染和牙周炎.
科学领域:
- 微生物学 微生物学
- 牙周内科医院 牙周内科医院 牙周内科医院
- 分子生物学分子生物学
背景情况:
- 初级内牙感染 (PEI) 和顶端牙周炎 (AP) 的特点是复杂的口腔细菌组.
- 了解微生物的组成和治疗的影响对于有效的内牙治疗至关重要.
研究的目的:
- 用PEI和AP研究牙中的细菌组.
- 量化和定性地评估使用2.5%的低化 (NaOCl) 的化学机械制剂 (CMP) 对这种细菌体的影响.
主要方法:
- 使用Illumina MiSeq平台从18名患者获得36个配对样本 (CMP前后) 的测序.
- 分析使用DADA2管道生成安普利康序列变异 (ASV) 的安普利康序列数据.
- 细菌负荷的定量PCR (qPCR),alpha多样性指标 (Chao1,Shannon,Simpson) 和PERMANOVA用于社区组成分析.
主要成果:
- 在CMP (s1) 之前,qPCR显示细菌数量显著增加,相比于 (s2) 之后,qPCR显示细菌数量显著增加.
- 香农和辛普森的多样性指数在CMP (s2) 后更高,表明微生物多样性的改变.
- 在s1和s2之间观察到社区组成的显著差异,在CMP之前丰富了Dialister和Prevotella等特定属,在CMP之后丰富了Actinomyces和Enterococcus.
结论:
- 带有PEI和AP的牙中的细菌组是复杂而多样化的.
- 含有2.5%NaOCl的CMP表明对减少内牙细菌组具有显著的定量和质量影响.
更多相关视频
相关概念视频
Modern Molecular Taxonomy
865
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
865
Applications of Molecular Taxonomy
717
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
717
Introduction to the Human Microbiota
222
Microorganisms colonize various regions of the human body, including the mouth, nasal passages, throat, stomach, intestines, urogenital tract, and skin. The total number of microbial cells is estimated to range from 10¹³ to 10¹⁴—comparable to, or exceeding, the number of human somatic cells. This host–microbiome relationship has led to the conceptualization of humans as supraorganisms, wherein microbial communities perform vital roles in development, immunity,...
222
The Oral Microbiota
97
The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...
97
Development of the Oral Microbiota
73
The establishment of the oral microbiome begins before birth, challenging the long-held belief that the fetal oral cavity is sterile. The presence of oral microbes such as Streptococcus and Fusobacterium in amniotic fluid suggests that microbial exposure may occur in utero, potentially through translocation from the maternal oral or gastrointestinal tract. This early colonization primes the neonatal immune system and sets the stage for subsequent microbial succession. Maternal health,...
73
Microbiota of the Urogenital Tract
59
The human urogenital system, once thought to be sterile in healthy individuals, is now recognized as a complex microbial habitat. Advancements in molecular sequencing techniques have revealed that even in healthy adults, the kidneys and bladder harbor microbial populations similar to those found in the distal urethra, albeit in much lower abundance. These resident microorganisms, while generally innocuous, can become opportunistic pathogens under conditions that alter the urogenital...
59


