石英晶微平衡作为一个整体检测器量化复杂有机矩阵在液体染色学: 2. 化合物特定同位素分析分析
Christopher Wabnitz1, Wei Chen1, Martin Elsner1
1Department of Chemistry, Chair of Analytical Chemistry and Water Chemistry, TUM School of Natural Sciences, Technical University of Munich, Lichtenbergstr. 4, 85748 Garching, Germany.
对环境微污染物的精确碳同位素分析需要样本净化. 将石英晶体微平衡 (QCM) 与HPLC相结合,可以增强矩阵去除,从而在较低度下进行精确的同位素测量.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 同位素分析的分析.
背景情况:
- 样品净化对于环境微污染物的精确碳化合物特定同位素分析 (碳CSIA) 至关重要.
- 自然有机物 (NOM) 可以干扰同位素完整性.
- 在净化过程中对矩阵的在线量化是必不可少的.
研究的目的:
- 调查QCM-HPLC对极性微污染物的同位素分析的好处.
- 研究NOM对同位素完整性的影响,并评估NOM-to-analyte比率作为纯度代理.
- 评估HPLC净化使用QCM对单个和多个目标的限制和增强.
主要方法:
- 石英晶体微平衡 (QCM) 与高性能液态色谱 (HPLC) 的合,用于在线矩阵量化.
- 研究NOM对模型分析物的同位素完整性的影响.
- 研究C18和C8阶段的HPLC净化,使用不同的NOM-to-analyte比.
- 选3到5种不同的HPLC梯度,以实现最佳的净化.
主要成果:
- 观察到强烈的同位素变化 (高达3.3%),NOM-to-analyte比率≥10.
- 对于晚泄化合物,QCM可实现高达99.8%的NOM去除.
- 同时净化多个目标降低了NOM清除效率 (高达2.5%).
结论:
- 使用QCM-HPLC优化HPLC净化可以显著提高碳CSIA准确性.
- 这种方法将准确分析的边界转移到较低的微污染物度 (高达两倍数).
- 系统的梯度选是实现同位素分析有效净化的关键.
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