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Updated: Apr 13, 2026

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
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在体外脂合成的协议,结合脂肪酸合成和无细胞基因表达

Rumie Matsumura1, Gaku Sato2, Yutetsu Kuruma1

  • 1Institute for Extra-cutting-edge Science and Technology Avant-garde Research (X-star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Natsushima-cho 2-15, Yokosuka 237-0061, Japan.

STAR protocols
|May 3, 2024
PubMed
概括

这项研究引入了一种新的无细胞系统,用于体外生产和量化脂. 这种方法简化了这些重要的生物分子的合成和分析,用于脂管学和合成生物学应用.

关键词:
生物技术和生物工程代谢学 代谢学 代谢学蛋白质表达和净化过程

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Synthesis of Phosphatidylcholine in the ER Membrane01:27

Synthesis of Phosphatidylcholine in the ER Membrane

The ER synthesizes lipids for building cell membranes and performing cellular functions such as energy storage and signaling. The lipid synthesis machinery embedded in the ER membrane primarily collects all reactants from the cytosol. Following synthesis, the secretory pathway and the ER contact sites distribute these lipids to other cellular organelles. Additionally, the energy-rich triacylglycerides are transported from the ER via lipid droplets.
The major components of all eukaryotic cell...
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