通过Arabidopsis ISOCHORISMATE SYNTHASE1进行chorismate异构的结构基础
Zihui Su1, Chengqun Niu1, Sicong Zhou1
1State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, Nanning 530004, P. R. China.
Plant physiology
|May 3, 2024
概括
植物异化合成酶1 (AtICS1) 酶通过依赖的异化催化酸 (SA) 前体的合成. 结构研究揭示了基质结合和封闭机制,与细菌酶共享.
科学领域:
- 生物化学 生物化学
- 植物分子生物学 植物分子生物学
- 结构生物学 结构生物学
背景情况:
- 酸 (SA) 对于植物对病原体的免疫力至关重要.
- 隔离体合成酶1 (AtICS1) 对于Arabidopsis thaliana中的SA生物合成至关重要.
- 植物同位素合成酶 (ICS) 的结构机制在很大程度上仍未被阐明.
研究的目的:
- 阐明植物ICS酶的结构和催化机制.
- 在AtICS1.1.中调查基质结合和识别.
- 为了比较植物ICS酶与细菌同类.
主要方法:
- 高分辨率的晶体学Apo AtICS1及其复合体与chorismate.
- 酶活性测试以确定催化效率.
- 与细菌的menaquinone, siderophore和tryptophan (MST) 酶进行结构比较.
主要成果:
- 植物ICS酶通过一种依赖于的机制催化胆质酸盐异构.
- 在AtICS1表现出显著的催化活性.
- 晶体结构揭示了基质结合,潜在的基质入口通道和封闭机制.
- 在AtICS1和细菌MST酶之间发现了结构上的相似性.
结论:
- 植物ICS酶利用保存的结构和门机制进行基质输送和催化.
- 这项研究为植物中SA生物合成的分子基础提供了关键的见解.
- 这些发现为了解和潜在地操纵植物防御途径铺平了道路.
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