在MDBK细胞系上通过表达由ivermectin诱导的DNA损伤响应基因对基因毒性作用的评估
Muhammad Muddassir Ali1, Zainab Farhad1, Muhammad Wasim1
1Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
PloS one
|May 3, 2024
概括
艾弗梅克丁 (IVM) 对麦丁-达比-牛-细胞表现出细胞毒性和基因毒性作用. 增加IVM度会增加DNA损伤标志物OGG1,这表明过度使用药物可能会造成危害.
科学领域:
- 毒理学 毒理学 毒理学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 伊弗梅克丁 (IVM) 是一种抗寄生虫药物,对其对SARS-CoV-2的潜在用途进行新兴研究.
- 在动物中过度使用IVM引起了对其毒理学特征的担忧.
- 以前的研究表明,IVM在动物模型中的潜在细胞毒性和基因毒性作用.
研究的目的:
- 为了研究甲 (IVM) 对麦丁-达比-牛- (MDBK) 细胞系的细胞毒性和基因毒性作用.
- 评估IVM对MDBK细胞中DNA损伤反应基因OGG1的表达的影响.
主要方法:
- 用MTT测定来评估细胞毒性.
- 基因毒性通过彗星和微核测试进行评估.
- 在RNA提取和cDNA合成后,使用qRT-PCR量化OGG1基因表达.
主要成果:
- 艾弗梅克丁在MDBK细胞上表现出剂量依赖的细胞毒性作用.
- 观察到基因毒性影响,由DNA损伤标志物证明.
- 随着IVM度的升级,OGG1基因表达的显著增加被注意到.
结论:
- 艾弗梅克丁在MDBK细胞系上表现出细胞毒性和基因毒性.
- 增加OGG1表达表明IVM诱导DNA损伤.
- 建议对IVM的分子毒性和对其他模型的影响进行进一步的研究.
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