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系统性长途硫运输及其在共生根结核蛋白循环中的作用

Alina Siegl¹, Leila Afjehi-Sadat², Stefanie Wienkoop³

¹Plant-Microsymbiont Interaction Lab, Division of Molecular Systems Biology, Department of Ecogenomics and Systems Biology, University of Vienna, Vienna, Austria; Research Support Facilities, Mass Spectrometry Unit UBB, University of Vienna, Vienna, Austria.

Journal of plant physiology

|May 3, 2024

概括

硫对植物营养和豆类共生至关重要. 这项研究表明,硫在系统上从芽向结节移动,即使在局部根硫酸盐缺乏的情况下,也确保了固定.

科学领域:

植物生理学植物生理学
植物与微生物的相互作用
营养物质运输营养物质运输

背景情况:

硫是一种必不可少的植物营养素,对豆类中固定共生至关重要.
系统硫运输在结节功能中的作用仍然不清楚.
硫的限制可能会对结节的发展和固定产生负面影响.

研究的目的:

为了研究在模型豆类Lotus japonicus中硫运输的系统调节.
为了确定硫酸盐吸收到结节是局部还是系统性的.
了解长距离的硫运输从芽到根到结节.

主要方法:

在Lotus japonicus中使用了分裂根系.
应用稳定同位素标记 (S) 来追踪硫的移动.
进行代谢物和蛋白质提取,然后进行质谱.

主要成果:

如果根系其他地方有足够的硫酸盐,局部硫酸盐缺乏不会影响结节的发展.
射击代谢物度没有表明显著的硫应激.
³⁴S同位素的丰富性证实了长距离的硫运输到结节.

结论:

硫通过植物的上部系统运输到结节.
硫分布到结节是需求驱动的,支持共生固定.
这种系统运输确保在不同的营养条件下保持结节功能.

关键词:

树生物的共生关系.根结节蛋白质组是一种根结节蛋白质组.分裂的根系系统.稳定同位素标签的标签硫酸盐运输是为了运输硫酸盐.系统性长途运输是系统性的长途运输.

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