针对细胞内泌尿病原性大肠杆菌菌株的珍特素载体
Jacopo Forte1, Linda Maurizi2, Maria Gioia Fabiano1
1Dipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, Piazzale Aldo Moro, 5, 00185, Rome, Italy.
Scientific reports
|May 3, 2024
概括
这项研究开发了新的纳米载体 (niosomes) 来向致尿道感染 (UTI) 的细菌细胞输送抗生素gentamicin. 这些体对改善抗生素输送和对抗持久的UPEC感染有前途.
科学领域:
- 纳米技术纳米技术
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 尿路感染 (UTI) 是一种常见的细菌感染,主要是由尿病原性大肠杆菌 (UPEC) 引起的.
- 由于药物透率差,UPEC在膀细胞内持久的能力给抗生素治疗带来了挑战.
- 细胞内UPEC感染很难用传统疗法消除.
研究的目的:
- 设计,准备和表征基于表面活性剂的纳米载体 (niosomes) 以提高抗生素的输送.
- 封装 gentamicin 在 niosomes 中,以克服其不良的细胞透.
- 为了评估体封装 gentamicin 对UPEC 在感染的膀细胞的疗效.
主要方法:
- 使用动态光散射,光谱学和传输电子显微镜进行了体体的表征.
- 确定了Gentamicin封装效率和释放动力学.
- 在T24膀细胞中使用Gentamicin保护试验和聚焦显微镜评估了体对UPEC入侵的影响.
主要成果:
- 体已经成功地准备好了,并以大小,z-潜力和稳定性进行了表征.
- 甘他素被有效地困在体内.
- 体治疗显著减少了UPEC在膀细胞中的入侵,这表明细胞内抗生素的释放.
结论:
- 基于表面活性剂的体是一种有前途的药物递送系统,可以增强抗微生物药物的内部化.
- 这种方法可以提供一种新的策略,以改善像UPEC引起的细胞内细菌感染的治疗.
- 进一步优化体可能会导致更有效的治疗持续性尿路感染.
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