产生具有复杂神经振荡的"半引导"皮质器官
Michael Q Fitzgerald1,2, Tiffany Chu2,3, Francesca Puppo2,3
1Department of Bioengineering, University of California San Diego, San Diego, CA, USA.
Nature protocols
|May 3, 2024
概括
这项研究产生了模仿人类大脑发育的成熟皮质器官. 这些有机体表现出神经振荡,为研究大脑发育和神经条件提供了一个新的模型.
科学领域:
- 神经科学是一个神经科学.
- 发育生物学 发展生物学
- 干细胞生物学 干细胞生物学
背景情况:
- 神经电生理学的发展反映了基因编程和细胞成熟.
- 神经振荡是反映大脑发育的新兴特性.
- 皮层有机体为研究人类大脑发育提供了一个模型.
研究的目的:
- 通过使用一种新的半导入协议,产生功能成熟的皮质器官.
- 描述这些有机体的电生理学特性.
- 建立一个可复制的模型来研究人类大脑发育和神经条件.
主要方法:
- 干细胞聚合成胚胎体,并用特定的介质培养,用于神经诱导,分化和成熟.
- 一个"半引导"的协议使用较短的诱导/分化步骤和特定的神经营养因子.
- 电生理学被描述为评估神经振荡成熟度.
主要成果:
- 半引导协议产生了多样化的细胞类型,类似于非引导方法,具有增强的可复制性.
- 机器人回顾了发育中的人类大脑中观察到的神经振荡的成熟.
- 该模型展示了发现人类大脑发育和神经疾病特征的潜力.
结论:
- 开发的半导体皮质器官协议成功模仿了人类大脑的电生理发育.
- 这些有机体是建模神经疾病和理解人类认知的宝贵工具.
- 这种方法是分子生物学,细胞生物学和人类认知研究之间的桥梁.
更多相关视频
14:192D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development
Published on: March 25, 2022
3.9K
05:40Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro
Published on: May 5, 2022
3.8K
相关概念视频
Somatosensation
The somatosensory system relays sensory information from the skin, mucous membranes, limbs, and joints. Somatosensation is more familiarly known as the sense of touch. A typical somatosensory pathway includes three types of long neurons: primary, secondary, and tertiary. Primary neurons have cell bodies located near the spinal cord in groups of neurons called dorsal root ganglia. The sensory neurons of ganglia innervate designated areas of skin called dermatomes.
Autonomic Nervous System: Overview
The human nervous system is divided into two main parts: the central nervous system (CNS) and the peripheral nervous system (PNS). The CNS is composed of the brain and spinal cord, while the PNS contains nerve cells, clusters of nerve cells, and the sensory receptors that are outside the CNS. The PNS has two types of nerve cells: sensory (afferent) and motor (efferent). Sensory cells send signals to the CNS from receptors, and motor cells carry signals from the CNS to organs, muscles, and...
Hierarchy of Motor Control
The hierarchy of motor control refers to the different levels of organization and processing involved in controlling movement in the body. These levels range from higher cortical areas involved in planning and decision-making to lower spinal cord reflexes that respond automatically to external stimuli.
Indirect Motor Pathways
The indirect motor or extrapyramidal pathways originate in the brainstem, the lower portion of the brain that connects it to the spinal cord. They consist of several distinct tracts, each with specialized functions. The four main tracts of the indirect motor pathways are the vestibulospinal tract, the reticulospinal tract, the tectospinal tract, and the rubrospinal tract.
The vestibulospinal tract originates in the vestibular nuclei of the brainstem. The vestibular system detects changes in...
The vestibulospinal tract originates in the vestibular nuclei of the brainstem. The vestibular system detects changes in...
Somatic Spinal Reflexes
Somatic spinal reflexes are rapid, involuntary muscular responses to external stimuli that involve the somatic musculature and the spinal cord.
One of the most well-known somatic spinal reflexes is the stretch reflex, which is activated by the sudden stretching of a muscle. This reflex involves the activation of specialized sensory receptors called muscle spindles, which are located in the muscle tissue and detect changes in the length and speed of muscle contractions. When a muscle is suddenly...
One of the most well-known somatic spinal reflexes is the stretch reflex, which is activated by the sudden stretching of a muscle. This reflex involves the activation of specialized sensory receptors called muscle spindles, which are located in the muscle tissue and detect changes in the length and speed of muscle contractions. When a muscle is suddenly...
Sympathetic Pathways: Sympathetic Chain Ganglia
The sympathetic chain ganglia, also known as the sympathetic trunk ganglia or paravertebral ganglia, are a series of ganglia located bilaterally on either side of the spinal column. These ganglia serve as relay stations for the sympathetic nervous system. Preganglionic neurons originating in the spinal cord project their axons to the sympathetic chain ganglia. Within the ganglia, these preganglionic fibers synapse with postganglionic neurons.The postganglionic neurons of the sympathetic trunk...
