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来自中性粒细胞的细胞外囊通过促进BMSCs的骨质分化来加速骨再生

Le Wang¹, Guanhua Zhang², Ye Gao¹

¹State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.

ACS biomaterials science & engineering

|May 4, 2024

概括

来自中性粒细胞 (PMN-EVs) 的细胞外囊泡显著促进骨髓中性干细胞 (BMSC) 的增殖和骨质分化. 将PMN-EV与BMSC细胞表集成,通过增强新的骨成熟,加速骨缺陷再生.

科学领域:

再生医学是一种再生医学.
生物材料科学生物材料科学
细胞生物学细胞生物学

背景情况:

骨缺陷重建面临着重大的全球挑战.
基于骨髓介质干细胞 (BMSC) 的细胞表显示出在体内骨质再生的前景.
细胞外囊泡 (EVs) 对于细胞间通信和组织修复至关重要.

研究的目的:

研究来自多态核白细胞 (PMN-EVs) 的EVs对BMSCs的影响.
探索PMN-EVs在骨再生中的潜在分子机制.
通过使用基于BMSC的细胞表来评估PMN-EVs在骨缺陷再生中的潜在应用.

主要方法:

开发与PMN-EV集成的基于BMSC的单元表 (BS@PMN-EV).
评估PMN-EVs对BMSC增殖和骨质原体分化体外的影响.
评估BS@PMN-EVs在加速老鼠骨缺陷中的骨再生in vivo.

主要成果:

在体外,PMN-EVs显著增强了BMSC的扩散和骨质原体的分化.
BS@PMN-EVs显著加快了大鼠骨缺陷中的骨再生和增强了新的骨成熟.
在BMSC中,PMN-EVs促进了关键骨质原蛋白 (SOD2,GJA1) 的表达.

结论:

PMN-EVs在促进BMSC骨质性差异化方面发挥着至关重要的作用.
这项研究提出了一种新的骨修复和再生策略,通过将PMN-EV与BMSC细胞表集成.
PMN-EVs代表了一种增强骨再生的有前途的治疗剂.

关键词:

骨再生骨再生细胞表格细胞表格细胞外囊泡中的细胞外囊泡.中性粒细胞中性粒细胞.骨质性分化的骨质性差异化

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Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...