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从带有DRaqL的组织段中获得高质量的单细胞RNA-seq的协议.

Hiroki Ikeda1, Shintaro Miyao1, Nanami Yamada1

  • 1Department of Embryology, School of Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan.

STAR protocols
|May 4, 2024
PubMed
概括

这项研究详细介绍了一种新的协议,DRaqL-Smart-seq2,用于从固定组织段中高质量的单细胞RNA测序. 它使单个细胞在其本土组织环境中进行全面的转录分析.

关键词:
发育生物学是发展生物学.在RNAseqqq中使用.一个单细胞单细胞.

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RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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