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在阳性血液培养中使用CLSI rAST的肠杆菌的断点

Jin Deng¹, YunHe An², Mei Kang¹

¹Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.

Diagnostic microbiology and infectious disease

|May 4, 2024

概括

快速抗微生物敏感性测试 (rAST) 可以在10小时内准确识别血液感染中的肠杆菌. 这种方法有助于为患有严重感染的患者及时做出临床决定.

科学领域:

临床微生物学临床微生物学
传染性疾病传染性疾病
抗微生物耐药性抗微生物耐药性

背景情况:

由肠杆菌引起的血流感染 (BSI) 需要快速的抗微生物敏感性测试 (AST) 才能有效治疗.
目前的AST方法可能耗时,延迟关键的治疗决策.
临床和实验室标准研究所 (CLSI) 开发了一种用于血液培养的直接快速抗微生物敏感性测试 (rAST).

研究的目的:

评估血液培养中的肠杆菌的CLSI rAST方法的准确性和速度.
为了将rAST结果与布鲁特大稀释法 (BMD) 作为基准进行比较.
评估早期检测碳烯耐药肠道细菌 (CRE) 的潜力.

主要方法:

直接快速抗微生物敏感性测试 (rAST) 应用于来自血液培养的常见肠道细菌.
使用rAST. 最低抑制度 (MICs) 的预测.
调料大稀释 (BMD) 的结果作为100%准确的基准.
数据使用分类一致 (CA),非常重大错误 (VME) 和重大错误 (ME) 来评估.

主要成果:

该CLSI rAST方法在10小时的时间内证明了其可靠性,在体外确定了肠道细菌对抗菌素的敏感性.
在分类中实现了90%的一致率.
确定了耐卡巴胺的肠道细菌 (CRE),在6小时,10小时和16小时的检测率没有显著差异,这表明最早6小时的检测潜力.

结论:

在临床环境中,CLSI rAST是快速检测肠杆菌对抗菌素敏感性的宝贵工具.
该测试在10小时内提供准确的结果,大大有助于治疗患有血液感染的患者.
通过这种方法,可以早期识别耐药性,包括CRE.

关键词:

抗菌药物是一种抗菌药物.血流感染血流感染这就是CLSI的CLSI.进入细菌体中的细菌.拉斯塔斯 (RAST) 是一个拉斯塔斯.

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