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Júlio Henrique Ferreira de Sá Queiroz¹, Tiago da Silva Ferreira¹, Bruno Fernandes Lima¹

¹Universidade Federal da Grande Dourados - UFGD, Laboratório de Pesquisa em Ciências da Saúde, Dourados, Mato Grosso do Sul, Brazil.

Diagnostic microbiology and infectious disease

|May 4, 2024

概括

这项研究在巴西的梅毒患者中检测到Treponema DNA,在两个样本中确定了阿齐思罗米辛耐药性. 发现了新的Treponema pallidum亚型,改善了该地区梅毒的特征.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
公共卫生公共卫生

背景情况:

梅毒是巴西重要的公共卫生问题.
关于巴西Treponema pallidum的遗传特征和抗生素耐药性的数据有限.

研究的目的:

在巴西梅毒患者的病变和血液样本中检测Treponema DNA.
描述巴西Treponema pallidum分离物和评估抗生素耐药性.

主要方法:

聚合酶链反应 (PCR) 和实时PCR (RT-PCR) 用于从18个病变和18个血液样本中放大Treponemal DNA.
巴西的分离品使用疾病控制和预防中心 (ECDC) 的基因定型方案进行分析,并选耐药基因.

主要成果:

通过RT-PCR检测到了8个病变和8个血液样本中的三重体DNA,通过PCR检测到了8个病变和3个血液样本中的三重体DNA.
两个样本显示对亚齐菌素的耐药性.
发现了14d/g,14d/c,15d/c和15d/e型的巴西分离物;14d/c,15d/c和15d/e型的亚型首次在巴西报告.

结论:

这项研究提供了巴西Treponema pallidum的关键分子数据.
鉴定了抗阿兹胺素耐药性和新型亚型,凸显了巴西持续监测和鉴定梅毒的必要性.

关键词:

电力发电中心 (ECDC) 计划这是一个PCRPCR.这是RT-PCR.三角膜DNA的三角膜DNA.这种基因是 polA 基因.

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