评估由马隆迪化物引起的蛋黄蛋白的结构和泡性质变化:实验和分子对接研究
Jingya Chen1, Jiaxin Jin1, Yu Liu1
1School of Food Science and Technology, Shihezi University, Xinjiang Uygur Autonomous Region, China; Key Laboratory of Agricultural Product Processing and Quality Control of Specialty (Co-construction by Ministry and Province), School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China; Key Laboratory for Food Nutrition and Safety Control of Xinjiang Production and Construction Corps, School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China.
马隆迪甲基 (MDA) 氧化改变了蛋黄蛋白 (EYP) 的结构和功能. 适度的MDA水平可以改善发泡特性,为黄皮产品的加工提供了洞察力.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 氧化压力是一种氧化压力.
背景情况:
- 蛋黄蛋白 (EYP) 对于食品应用至关重要.
- 氧化性修饰可以显著影响蛋白质的功能.
- 了解马隆迪化物 (MDA) 的作用对于食品加工至关重要.
研究的目的:
- 为了研究马隆迪甲基 (MDA) 对蛋黄蛋白 (EYP) 结构和发泡特性的影响.
- 探索MDA和EYP之间的分子相互作用.
- 为在加工过程中管理黄黄产品质量提供见解.
主要方法:
- 具有不同MDA水平的EYP的氧化修饰.
- 对蛋白质碳和硫含量的分析.
- 测量EYP溶解度,粒子大小和泽塔电位.
- 二甲基硫酸盐 - 聚烯胺凝电泳 (SDS-PAGE).
- 分子对接模拟. 分子对接模拟.
主要成果:
- MDA增加了EYP碳酸含量 (4.49倍),并降低了硫酸含量 (21.47%).
- 随着氧化,EYP的溶解度显著降低.
- 氧化导致了蛋白质的交联,聚合,并改变了粒子大小/泽塔潜力.
- 适度的MDA水平 (<1 mmol/L) 提高了发泡能力和稳定性.
- 分子对接揭示了MDA和EYP之间的有利相互作用 (键).
结论:
- 马隆迪甲基 (MDA) 诱导卵黄蛋白 (EYP) 的结构变化.
- 适度的MDA诱导氧化可以有益地影响EYP发泡特性.
- 调查结果有助于控制黄产品的功能和质量属性.
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