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Updated: Jun 27, 2025

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

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开发一种实时光反转录循环介导的同热放大试验,使用灭原料,用于快速检测红疹病毒

Kiyoko Okamoto1, Kazue Kadosawa1, Rieko Suzuki2

  • 1Center for Emergency Preparedness and Response, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.

Journal of virological methods
|May 4, 2024
PubMed
概括

一种新的快速红疹检测试验显著缩短了诊断时间. 这种方法提供了一个更快,更有效的工具,用于在特有地区识别红疹病毒.

关键词:
诊断 诊断 诊断 诊断 诊断反转录循环介导的同热放大试验.红疹是一种红疹.

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Reverse Transcription-Loop-mediated Isothermal Amplification RT-LAMP Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples

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Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings09:36

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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