通过Yersinia效应器进行罗斯因修饰的糖化
Silvia Schneider1, Christophe Wirth2, Thomas Jank1
1Faculty of Medicine, Institute for Experimental and Clinical Pharmacology and Toxicology, University of Freiburg, Freiburg, Germany.
The Journal of biological chemistry
|May 4, 2024
概括
两种Yersinia glycosyltransferases,YeGT和YkGT,通过将GlcNAc添加到氨酸残留物中来修改宿主Rho蛋白. YkGT显示出比YeGT更广泛的基质特异性,影响宿主细胞功能.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- 细菌葡萄糖转移酶是修改宿主蛋白质的关键毒性因子.
- 毒素的单-O-糖化干扰宿主细胞的功能.
- 耶尔西尼亚物种具有具有高序列身份的甘氨酸转移酶.
研究的目的:
- 为了描述两种Yersinia糖酶转移酶,YeGT和YkGT.
- 为了研究它们的基质特异性和结构性质.
- 了解它们在宿主细胞修饰和毒性中的作用.
主要方法:
- 序列分析和YeGT和YkGT的比较.
- 酶分析以确定基质特异性 (Rho家族蛋白质).
- 位点定向突变发生,以确定关键残留物 (例如,残留物177).
- 截断的YeGT (sYeGT) 的晶体结构的确定.
- 使用炭毒素进行蛋白质转位和细胞成像的功能性测试.
主要成果:
- 在氨酸残留物 (RhoA中的Tyr-34) 处的YeGT和YkGT糖酸Rho家族蛋白与GlcNAc.
- YeGT的目标是RhoA,B和C,而YkGT的范围更广,包括Rac和Cdc42.
- 其余177对于YkGT更广泛的基质特异性至关重要.
- sYeGT 的晶体结构将其置于GT-A家族中,类似于毒素甘氨酸转移酶.
- N-终端16残留物对于转移到宿主细胞至关重要.
- 对YeGT/YkGT的表达会诱导宿主细胞的形态变化和actin细胞骨架的改变.
结论:
- YeGT和YkGT是细菌效应蛋白,属于细菌糖化转化酶家族的铁糖化转化酶.
- 它们的糖化活性和转位机制有助于Yersinia的毒性.
- 与已知毒素的结构和功能相似性突出显示了细菌病原体的融合进化.
关键词:
克洛斯特里迪奥伊德斯困难菌这种毒素是Photorhabdus asymbiotica的毒素.对于rho GTPases来说,这是一个很好的方法.这种植物是Yersinia enterocolitica.耶尔西尼亚克里斯尼亚克里斯尼亚耶尔西尼亚·鲁克里 (Yersinia ruckeri) 是一种植物.葡萄糖转移酶的使用方法毒素毒素是一种毒素.铁氨酸的糖化化.更多相关视频
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