在复发性和非复发性结肠癌中编码和非编码RNA的转录格局
Ulrik Korsgaard1, Juan L García-Rodríguez2, Theresa Jakobsen2
1Department of Clinical Pathology, Vejle Hospital, Vejle, Denmark; Danish Colorectal Cancer Center South, Vejle Hospital, Vejle, Denmark.
The American journal of pathology
|May 4, 2024
概括
研究人员确定了结肠癌复发的新型RNA生物标志物. 他们发现特定的小核RNAs (snoRNAs) 和伪基因在晚期瘤中显著上调,提供了新的预后潜力.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 基因组学就是基因组学.
背景情况:
- 患有局部或局部晚期疾病的结肠癌患者通常会出现复发.
- 需要改善预后生物标志物来预测这些患者的复发.
- 目前对结肠癌复发中的转录基因情景的理解尚不完整.
研究的目的:
- 综合描述结肠癌的转录基因格局,重点关注各种RNA类.
- 识别与瘤复发相关的新型差异表达RNA转录.
- 评估新发现的结肠癌转录的预后生物标志物潜力.
主要方法:
- 总RNA测序用于描述mRNA,长非编码RNA,snRNA,小核核RNA (snoRNA),小Cajal体特异RNA,伪基因和圆形RNA.
- 进行了差异表达分析,以比较复发性和非复发性结肠癌之间的RNA概况.
- 使用接收器操作特征 (ROC) 分析来评估已识别的转录的预后潜力.
主要成果:
- 许多差异表达的RNA转录,包括以前未被描述的转录 (例如,RNA5SP149,RNU4-2,SNORD3A),在复发性和非复发性结肠癌之间被确定.
- 在更先进的瘤中观察到snRNA伪基因,snoRNA和rRNA伪基因的全球上调.
- 与正常组织相比,在瘤中观察到循环RNA的全球下调.
结论:
- 这项研究揭示了在结肠癌复发中跨多个类别的多样性表达RNA的多样性.
- 在晚期瘤中,上调的snRNA假基因,snoRNA和rRNA假基因代表了重要的发现.
- 一些新型RNA候选物,如RNU6-620P,RNU2-20P,VTRNA1-3和RNA5SP60,显示出强大的预后潜力,特别是在II期结肠癌中.
更多相关视频
03:37Author Spotlight: Impact of Intergenic Interactions on Disease-Identifying Dark Biomarkers
Published on: March 1, 2024
704
09:16Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
Published on: September 1, 2019
7.6K
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
03:18Non-LTR Retrotransposons
11.5K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.5K
01:37Epigenetic Regulation
3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
3.0K
02:02Cis-regulatory Sequences
9.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.9K
01:33Cancer-Critical Genes I: Proto-oncogenes
8.8K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.8K
02:24Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K