脱化坐标压缩器功能用于感染大米爆发真菌
Hong Hu1, Wenhui He1, Zhiguang Qu1
1National Key Laboratory of Agricultural Microbiology and Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
概括
大米爆发真菌需要去化,才能形成压缩物. S-酸还原酶 (GSNOR) 调节了这一过程,通过破坏氧化 (NO) 稳态来突出潜在的真菌疾病控制策略.
科学领域:
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
- 植物病理学 植物病理学
背景情况:
- 氧化 (NO) 是一个关键的信号分子,调节发育和应激反应.
- 蛋白质S-化,一个关键的NO活动,在真菌病原发生过程中没有明确的作用.
- 在Magnaporthe oryzae中形成的压缩体对于大米感染至关重要.
研究的目的:
- 为了研究脱化在Magnaporthe oryzae中的appressorium形成中的作用.
- 为了识别通过S-化和脱化调节的蛋白质.
- 探索菌疾病控制的潜在策略,以向NO稳态.
主要方法:
- 使用一个indoTMT开关标记蛋白质组学技术.
- 分析了对appressorium形成至关重要的蛋白质中的S-化位.
- 研究了破坏NO平衡的影响 (使用NO捐赠体,食尸体和GSNOR抑制剂).
主要成果:
- 脱化对于Magnaporthe oryzae中的功能性压缩体形成至关重要.
- 通过GSNOR介导的脱化减轻了化应激,并促进了感染.
- 在483种蛋白质上确定了741个S-化位,包括Mgb1,MagB,Sps1,Cdc42和septins等关键的压缩蛋白.
- 由GSNOR介导的S-化位移除对蛋白质结构和压缩功能至关重要.
结论:
- 通过GSNOR介导的脱化是Magnaporthe oryzae中appressorium形成的关键调节者.
- 通过NO的捐赠者,食尸动物或GSNOR抑制剂来破坏NO的稳态,为控制真菌病提供了有前途的途径.
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