血清外体tRFs作为活跃结核病和潜伏结核病感染的有希望的生物标志物
Xiangyu Xi1, Binghua Wang2, Ruimei Zhang3
1The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China; Xuzhou Infectious Disease Hospital, Xuzhou, Jiangsu, China.
Journal of microbiological methods
|May 5, 2024
概括
血清外体tRNA片段 (tRFs) 和微小tRNAs (tiRNAs) 在活跃结核病 (TB) 和潜伏结核病感染中显示出不同的表达特征. 这些分子作为诊断结核病活动的有希望的生物标志物.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 结核病 (TB) 诊断依赖于各种方法,但区分活跃疾病与潜伏感染可能具有挑战性.
- 外体,小的细胞外囊泡,携带核酸,包括tRNA衍生碎片 (tRFs) 和微小的tRNAs (tiRNAs),可能反映疾病状态.
研究的目的:
- 分析血清外体tRFs/tiRNAs的表达特征.
- 评估它们在区分活跃结核病 (ATB) 与潜伏结核病感染 (LTBI) 和健康对照 (HCs) 的诊断价值.
主要方法:
- 从ATB患者,LTBI患者和HC患者的血清外体tRFs/tiRNAs的高通量测序.
- 使用定量实时聚合酶链反应 (qRT-PCR) 验证选定的tRFs/tiRNAs.
- 使用接收器操作特征 (ROC) 曲线分析和曲线下的面积 (AUC) 进行诊断性能评估.
- 生物信息学分析以确定潜在的生物途径.
主要成果:
- 在ATB,LTBI和HC组中观察到明显的血清外体tRF/tiRNA表达特征.
- 与对照组相比,ATB患者的tRF-1-22-chrM.Ser-GCT的表达是上调的,而tRF-56:75-Trp-CCA-4的表达是下调的.
- 对tRF-56:75-Trp-CCA-4和tRF-1:22-chrM.Ser-GCT的AUC值分别为0.824和1.000,表明具有显著的诊断价值.
- 这些tRF有效地区分了ATB和LTBI患者.
结论:
- 血清外体tRFs/tiRNAs表现出与结核病活动相关的差异性表达模式.
- 特定的tRFs,即tRF-56:75-Trp-CCA-4和tRF-1:22-chrM.Ser-GCT,显示出作为诊断活跃结核病和区分其与LTBI的非侵入性生物标志物的潜力.
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