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年龄对眼腺生物活性脂质的影响

Brandon Ebright¹, Zhiyuan Yu², Priyal Dave¹

¹Department of Clinical Pharmacy, Alfred Mann School of Pharmacy and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA, United States, 90089-9121, USA.

The ocular surface

|May 5, 2024

概括

衰老减少了多不和脂肪酸 (PUFA) 和它们的代谢物在老鼠的眼腺 (LGs),增加了炎症风险. 这项研究突出显示了LG群体随着年龄的增长而发生的PUFA代谢变化.

科学领域:

利皮多米克 (Lipidomics) 是一种消化剂.
炎症研究的研究.
生物老龄化生物学

背景情况:

多不和脂肪酸 (PUFA) 是关键的生物活性脂质,参与调节炎症及其解决.
脂质新陈代谢的失调与与年龄有关的炎症状况有关.

研究的目的:

为了研究老年相关的变化PUFA代谢在老鼠眼腺 (LGs).
为了比较年轻和老年C57BL/6 J小鼠LGS之间的PUFA概况和酶活性.

主要方法:

向性脂组学试验用于量化PUFA及其代谢物在年轻小鼠和老鼠LGS中的量化.
参与PUFA代谢的酶的基因表达分析.
代谢物比率作为酶活性的替代措施.

主要成果:

在老年LGs中观察到PUFAs和代谢物的总体减少 (25-66%).
欧米茄-6阿拉基酸 (AA) 的代谢物显著降低,包括前列腺素E2 (PGE2) 和素A4 (LXA4).
降低了omega-3 docosahexaenoic acid (DHA) 和eicosapentaenoic acid (EPA) 的代谢物水平,同时降低了12-lipoxygenase (12-LOX) 的活性.

结论:

与年轻对照对照相比,老鼠的LG显示PUFA及其代谢物的总体显著减少.
这种新陈代谢转变,除了多科萨氨酸 (DPA) 和其代谢物外,表明在老化过程中对眼腺炎症的易感性增加.
这些发现表明,改变PUFA代谢和与年龄相关的眼表面疾病之间存在潜在的联系.

关键词:

衰老的衰老衰老的衰老眼睛干燥眼睛干燥眼腺是眼腺的一种.脂质脂质脂质是一种

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