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相关概念视频

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
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相关实验视频

Updated: Jun 27, 2025

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
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Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

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分子细胞测量用于全面的免疫分析.

Pratip K Chattopadhyay1

  • 1Talon Biomarkers, Whippany, NJ, United States.

Methods in cell biology
|May 5, 2024
PubMed
概括
此摘要是机器生成的。

分子细胞计,一个高参数单细胞分析技术,同时测量蛋白质和转录. 这种强大的免疫监测工具为细胞功能和疾病提供了深入的见解.

关键词:
生物标志物 生物标志物细胞计量 (Cytometry) 是一种细胞计量方法.免疫监测监测 免疫监测测序测序是指测序的时间.

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Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
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相关实验视频

Last Updated: Jun 27, 2025

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
12:36

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

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Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
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Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

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Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR

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科学领域:

  • 单细胞生物学 单细胞生物学
  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学

背景情况:

  • 高参数单细胞分析对于理解复杂的生物系统至关重要.
  • 现有的技术在同时测量蛋白质和转录水平方面存在局限性.
  • 免疫监测需要详细的细胞表征.

研究的目的:

  • 描述分子细胞测量技术平台.
  • 为优化分子细胞测量实验提供指导.
  • 讨论分子细胞计在研究中的应用和潜力.

主要方法:

  • 使用带有寡核酸标记的抗体进行蛋白质检测.
  • 结合蛋白质测量与向基因或整个转录组分析.
  • 使用中等通量单细胞分析 (10-100K细胞).

主要成果:

  • 该平台可以同时测量100多种蛋白质和数百种基因或整个转录组.
  • 证明了分子细胞计量用于免疫监测的力量.
  • 突出了分子细胞计量研究的应用和潜在见解.

结论:

  • 分子细胞计量 (包括CITE-Seq,REAP-Seq,AbSeq) 是单细胞分析的强大工具.
  • 优化指导和对局限性的理解对于有效使用至关重要.
  • 这项技术为推进免疫监测和生物研究提供了巨大的潜力.