度生物测试:一种解决方案,用于检测无症状细菌尿症与尿病原性大肠杆菌 (Escherichia coli) 隔离物中的抗菌活性
Ciara Kenneally1, Craig P Murphy1, Roy D Sleator1
1Department of Biological Sciences, Munster Technological University, Bishopstown, Cork, Ireland.
MicrobiologyOpen
|May 6, 2024
概括
无症状细菌尿症分离物产生细菌,抑制尿病原性大肠杆菌. 这些在人工尿中增强的细菌素显示出对抗生素耐药菌株的新疗法的潜力.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 传统的细菌素查方法受限于的扩散问题.
- 度测量技术克服了扩散限制,并量化了细菌素的有效性.
- 无症状细菌尿症 (ABU) 隔离物是新型抗菌剂的潜在来源.
研究的目的:
- 选ABU分离物和埃舍里希亚大肠杆菌83972对抗尿病原性大肠杆菌 (UPEC) 的抗菌活性.
- 描述产生细菌素的ABU分离物.
- 调查ABU细菌素作为抗生素耐药UPEC的治疗药物的潜力.
主要方法:
- 从ABU分离物和大肠杆菌83972中获取的无细胞超体 (CFS) 用度增长方法对UPEC菌株进行了选.
- 积极的CFS被测试为蛋白酶K的抑制,以确认蛋白质性质.
- 进一步鉴定出产生细菌素的分离物,并在人工尿媒介中评估它们的活性.
主要成果:
- 八个ABU分离物和大肠杆菌83972被选;几个显示出针对UPEC的显著抗菌活性.
- 蛋白酶K抑制了抗微生物活性,这表明它具有类似细菌素的性质.
- 大肠杆菌PUTS 58和SK-106-1在人工尿中表现出增强的活性,抑制了所有测试的UPEC菌株.
- 基因分析确定了大肠杆菌SK-106-1中的微氨酸H47操作子,PUTS 37中的微氨酸V,PUTS 58中的胆氨酸E7.
结论:
- ABU隔离物具有强大的抗UPEC的细菌素生产能力.
- 在模仿尿路的条件下,细菌素活性可以增强.
- ABU的细菌素生产者代表了开发针对抗生素耐药性尿病原体的新型预防和治疗策略的有希望的候选人.
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