Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Experimental RNAi02:15

Experimental RNAi

6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

16.7K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.7K
RNA Interference01:23

RNA Interference

26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

3.5K
3.5K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

893
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
893
MicroRNAs01:22

MicroRNAs

3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Association of Early Supplemental Parenteral Nutrition with Clinical Outcomes in Patients with Traumatic Brain Injury.

Journal of Korean Neurosurgical Society·2026
Same author

Analysis of the Epidemiological Characteristics and Public Health Response to Healthcare Facility-associated Measles Outbreaks in the Honam Region, Republic of Korea, 2025.

Public health weekly report·2026
Same author

Adoption of artificial intelligence in drug review across the lifecycle: Transformation of regulatory decision-making.

Regulatory toxicology and pharmacology : RTP·2026
Same author

Medium-chain inulin reshapes the gut microbiome-metabolome axis to counteract high-fat diet-induced obesity.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

Astrocyte-microglia crosstalk unlocks Alzheimer's disease.

Immunity·2026
Same author

Stage-specific polysomnographic and MRI markers across phenoconversion in isolated REM sleep behavior disorder.

Journal of neurology·2026

相关实验视频

Updated: Jun 27, 2025

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System
14:55

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

Published on: May 12, 2013

60.3K

ASOptimizer:通过深度学习优化IDO1基因调节的反意义寡核酸.

Gyeongjo Hwang1, Mincheol Kwon2, Dongjin Seo1

  • 1Spidercore Inc, 17, Techno 4-ro, Yuseong-gu, Daejeon 34013, South Korea.

Molecular therapy. Nucleic acids
|May 6, 2024
PubMed
概括

一个新的深度学习平台,ASOptimizer,有效地设计反意义寡核酸 (ASOs) 以准促进癌症的基因,如IDO1. 这项技术加速了新型RNA向疗法的开发,提高了疗效和安全性.

关键词:
吉布斯的自由能量是自由的.MT: 生物信息学 生物信息学这是一种反意义的寡核酸.化学修饰是一种化学修饰.深度学习是一种深度学习.盖普梅尔的差距更大.分子图谱 分子图谱优化RNA药物的使用.双向学习是双向学习.二级结构是二级结构的二次结构.

更多相关视频

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
10:21

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

8.4K
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

9.6K

相关实验视频

Last Updated: Jun 27, 2025

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System
14:55

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

Published on: May 12, 2013

60.3K
Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
10:21

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

8.4K
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

9.6K

科学领域:

  • 生物技术是生物技术.
  • 计算生物学 计算生物学
  • 癌症治疗方法 癌症治疗方法

背景情况:

  • 反感性寡核酸 (ASO) 对RNA调节有前途,包括准以前无法药物治疗的分子.
  • ASO的手工设计是劳动密集型的,阻碍了更广泛的应用.
  • 氨酸2,3-二氧化酶1 (IDO1) 是癌症生存中的关键酶,在瘤微环境中促进免疫抑制.

研究的目的:

  • 开发一个经济高效的,基于深度学习的框架,ASOptimizer,以实现高效的ASO设计.
  • 识别和优化针对潜在癌症治疗的IDO1mRNA的ASO.

主要方法:

  • ASOptimizer采用一个两阶段的过程:序列工程和化学工程.
  • 序列工程优化ASO序列,以实现高效的mRNA向.
  • 化学工程精炼ASO以增强抑制活性和降低细胞毒性.

主要成果:

  • ASOptimizer 成功识别了针对 IDO1 mRNA 的 ASO 序列.
  • 该平台优化了化学修改,以提高ASO性能.
  • 设计的ASO证明了提高效率和安全的潜力.

结论:

  • ASOptimizer是一个强大的工具,可以加速设计有效和安全的ASO.
  • 这个平台可以促进新型RNA向疗法的开发.
  • 该研究强调了ASOptimizer在针对IDO1.1的癌症治疗策略中的潜力.