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使用基于AFM的技术,洞察纤维素纳米纤维和Matrigel水凝中的球形形成

Roberta Teixeira Polez^1,2, Ngoc Huynh¹, Chris S Pridgeon^1,2

¹Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, FI-00076, Aalto, Finland.

Materials today. Bio

|May 6, 2024

概括

细胞-生物材料相互作用影响3D细胞模型的形成. 纤维素纳米纤维素通过对E-和N-cadherins进行上调来促进紧的球体,与Matrigel不同,为药物研究提供了洞察力.

科学领域:

生物材料科学生物材料科学
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

监管的转变有利于体外模型,增加了对先进的细胞培养技术的需求,如球体.
了解细胞矩阵相互作用对于选择适当的生物材料以支架为基础的3D细胞培养至关重要.
目前关于环境对球形形成的影响的知识仍然不完整.

研究的目的:

用原子力显微镜研究细胞-生物材料相互作用在球形形成中的作用.
为了比较 Matrigel 和纤维素纳米纤维 (CNF) 上的细胞粘附和细胞-细胞力.
阐明各种生物材料中球形形态差异背后的分子机制.

主要方法:

使用原子力显微镜 (AFM) 来量化细胞对Matrigel和CNF的粘附力.
在肝细胞癌 (HepG2) 和诱导多能干细胞 (iPS(IMR90) -4) 中测量了细胞-细胞粘附力.
分析了细胞粘附蛋白的基因表达 (E-cadherin,N-cadherin) 和整合素-氨酸相互作用.

主要成果:

无论是 HepG2 和 iPS (IMR90) - 4 细胞,都在 CNF 上形成了紧的球形,但在 Matrigel 上形成了松散的聚合物.
细胞类型特定的粘附蛋白表达,不仅仅是键强度,指定的球形紧度.
CNF培养上调了E和N-cadherin的表达,促进了紧的球状体的形成.
马特里格尔培养导致整合素-氨酸结合和下调的E-cadherin,从而产生更松的聚合物.

结论:

细胞-生物材料相互作用显著影响3D细胞模型的开发和形态学.
表达的细胞粘附分子类型是球形形成的关键决定因素,比粘附强度更重要.
这些发现为设计改进的生物材料和3D细胞模型提供了基础,用于药物发现和疾病研究.

关键词:

原子力显微镜的原子力显微镜.细胞粘附分子的细胞粘附分子细胞相互作用细胞相互作用纤维素纳米纤维的纤维素.马特里格尔 (Matrigel) 是一种孕产妇用品.球形形成的球形形成.

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