长非编码RNAs热冲击RNAomega核化TBPH,并在热冲击时促进肠道干细胞的分化
Yinfeng Guo1, Meng Wang1, Jiaxin Zhu1
1State Key Laboratory of Genetic Engineering, Department of Cell and Developmental Biology at School of Life Sciences, Institute of Metabolism and Integrative Biology, Human Phenome Institute, Department of Liver Surgery and Transplantation of Liver Cancer Institute at Zhongshan Hospital, Fudan University, Shanghai 200438, China.
iScience
|May 6, 2024
概括
长非编码RNA Hsrω形成omega斑点,促进Drosophila的肠干细胞分化. 这种由热冲击引起的过程在哺乳动物细胞中得到保存,为干细胞分化提供了新的策略.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
背景情况:
- 在热冲击下,长非编码RNA Hsrω在Drosophila中形成omega斑点.
- 由Hsrω诱导的欧米茄斑块的生物功能仍然在很大程度上是未知的.
研究的目的:
- 为了研究成年Drosophila组织中欧米茄斑点的分布和功能.
- 阐明Hsrω和omega斑点在肠干细胞 (ISC) 分化中的作用.
- 探索哺乳动物系统中热冲击诱导的细胞分化机制的保存.
主要方法:
- 对成年Drosophila melanogaster的欧米茄斑点分布的组织特异分析.
- 实验操纵Hsrω表达 (过度表达和热冲击) 以诱导欧米茄斑块组装.
- 研究TBPH (暂时表达的TDP-43结合蛋白) 核和ISC分化.
- 对哺乳动物细胞分化的热冲击效应进行比较分析,包括人类诱导的多能干细胞 (hiPSCs).
主要成果:
- 欧米茄斑点被选择性地发现在分化的肠细胞中,而不是在Drosophila midgut的肠道干细胞中.
- 高Hsrω表达或强烈的热冲击诱导了欧米茄斑块组合,它核化了TBPH并促进了ISC分化.
- 热冲击应激诱导了哺乳动物细胞的细胞分化,通过斑形成和TDP-43 (TBPH同源) 聚合在hiPSCs中证明了这一点.
结论:
- 和其组装的欧米茄斑点在Drosophila的肠道细胞发育和分化中起着至关重要的作用.
- 这些发现表明,热冲击诱导的细胞分化的保存机制涉及Hsrω/omega斑点和TBPH/TDP-43.
- 这项研究为操纵干细胞分化提供了新的见解和潜在策略.
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