发现脊髓损伤和骨关节炎之间的神经免疫相关的共同调节机制
Yuxin Zhang1,2,3, Dahe Zhang2, Xin Jiao3
1Department of Rehabilitation Medicine, Fengcheng branch, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
Heliyon
|May 6, 2024
概括
脊髓损伤 (SCI) 和骨关节炎 (OA) 具有共同的分子路径. 这项研究确定了一个miRNA-gene-pathway网络,突出了CD44,TGFBR1,CCR5和IGF1作为这两种疾病的潜在生物标志物.
科学领域:
- 神经免疫学 神经免疫学
- 生物信息学是一种生物信息学.
- 基因组学就是基因组学.
背景情况:
- 脊髓损伤 (SCI) 的成年人表现出较高的骨关节炎 (OA) 发病率.
- 了解SCI和OA之间共享的神经免疫调节机制对于开发有效的治疗方法至关重要.
研究的目的:
- 通过生物信息学分析识别常见的差异表达基因 (DEG),微RNA (miRNA) 和涉及SCI和OA的信号通路.
- 构建一个连接这些分子实体的监管网络,并验证关键参与者.
主要方法:
- 来自基因表达综合 (GEO) 数据库的微阵列数据的生物信息分析.
- 差异表达基因 (DEGs) 的查和蛋白质-蛋白质相互作用 (PPI) 的构建以及权重基因共表达网络分析 (WGCNA) 网络.
- 识别共享的miRNA和构建一个miRNA-基因通路调节网络,然后进行RT-qPCR验证.
主要成果:
- 在SCI和OA样本中确定了185种常见的DEG.
- 建立了一个涉及五个基因 (CD44,TGFBR1,CCR5,IGF1),六个miRNA和六个信号通路的调节网络.
- 在两种条件下,RT-qPCR证实了CD44,TGFBR1,CCR5和IGF1的上调和特定miRNAs (miR-125b-5p,miR-130a-3p,miR-16-5p,miR-204-5p,miR-204-3p) 的下调.
结论:
- 一个共享的miRNA-基因通路网络是SCI和OA的神经免疫机制的基础.
- 已识别的基因 (CD44,TGFBR1,CCR5,IGF1) 和miRNAs代表了SCI和OA的潜在诊断生物标志物和治疗标.
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