达成奇可以改善严重的急性胰腺炎毛细血管泄漏综合征,通过调节紧密结合相关蛋白质
Jiamin Tu1, Yinping Jiang2, Lei Tu1
1Department of Proctology, University of Chinese Academy of Science Shen Zhen Hospital (GuangMing), ShenZhen, China.
Frontiers in pharmacology
|May 6, 2024
概括
达成奇 (Da-Cheng-Qi) (DCQD) 通过改善毛细血管的透性来缓解严重的急性胰腺炎 (SAP). 它可以调节像claudin 5,ocludin和ZO-1这样的紧结蛋白,从而减少SAP诱导的 in vivo 和 in vitro 损伤.
科学领域:
- 胃肠病学和肝病学
- 药理学和毒理学 药理学和毒理学
- 细胞生物学 细胞生物学
背景情况:
- 严重的急性胰腺炎 (SAP) 是一种由毛细血管泄漏综合征特征的严重疾病.
- 了解传统中医药的治疗机制,如达成 (DCQD),对于管理SAP至关重要.
- 毛细管的透性是SAP的一个关键病理特征.
研究的目的:
- 阐明DCQD如何影响SAP中的毛细血管泄漏的机制.
- 在SAP模型中研究DCQD对紧结蛋白表达的影响.
- 在体内和体外SAP模型中评估DCQD的治疗潜力.
主要方法:
- 建立了使用陶酸 perfusion 的SAP 的老鼠模型.
- 与SAP诱导相比,在指定的时间点通过 gavage 管理DCQD.
- 使用高性能液态染色学 (HPLC) 分析DCQD组件.
- 评估了胰腺组织病理,毛细血管的透性和炎症标志物 (氨酶,TNF-α,IL-1β,IL-2,IL-6).
- 使用qRT-PCR,免疫光学和西方斑点的密切结合蛋白 (claudin 5,ocludin,ZO-1,JAM-C) 的量化mRNA和蛋白质表达.
- 研究了DCQD对人类静脉内皮细胞 (HUVEC) 的影响,以评估体外毛细血管透性和蛋白质表达.
主要成果:
- 与SAP组相比,DCQD治疗显著降低了血清氨酶和炎症性细胞因子水平.
- 在SAP大鼠中,DCQD减轻了胰腺组织损伤和毛细血管透率的降低.
- 在胰腺组织中,DCQD上调了claudin 5,ocludin和ZO-1的表达,同时降低了JAM-C的表达.
- 实验室研究表明,DCQD改善了HUVEC的透性,并上调了Claudin 5,Occludin和ZO-1的表达.
结论:
- 在体内和体外SAP模型中,DCQD有效地提高了毛细管的透性.
- DCQD的治疗效果与克劳丁5,奥克卢丁和ZO-1表达的上调有关.
- 通过稳定内皮屏障功能,DCQD显示出作为SAP治疗的潜力.
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