与酶相关的脂质纳米载体,用于应对伪冠肺部感染. 纳米载体是否会补充生物膜破坏或铺平其道路?
Noha Nafee1,2, Dina M Gaber3, Alaa Abouelfetouh4,5
1Department of Pharmaceutics, College of Pharmacy, Kuwait University, Safat, 13110, Kuwait.
International journal of nanomedicine
|May 6, 2024
概括
新型固体脂质纳米颗粒 (SLN) 装有质量检测抑制剂和酸酶,有望治疗囊性纤维化患者的持久性Pseudomonas aeruginosa生物膜. 需要进一步的研究才能充分理解纳米载体-生物膜相互作用.
科学领域:
- 纳米技术 纳米技术
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 囊性纤维化 (CF) 的特点是持续的肺部感染,通常是由抗生素耐药的*Pseudomonas aeruginosa*引起的.
- 细菌生物膜,特别是P. aeruginosa生物膜,在CF治疗中构成重大挑战,原因是它们固有的抗生素耐药性.
研究的目的:
- 开发和评估新型固体脂质纳米颗粒 (SLNs),用于针对性地消灭囊性纤维化中的*Pseudomonas aeruginosa*生物膜.
- 调查定数感知抑制剂 (QSI),酸盐涂层和酸盐酶固定对SLN对细菌生物膜的疗效的协同作用.
主要方法:
- 固体脂质纳米颗粒 (SLNs) 被配制并装载了一个定数感应抑制剂 (QSI).
- SLN被涂上酸盐 (CS) 来增强细胞吸收,并用酸盐酶 (AL) 固定来降解生物膜基质.
- 开发的纳米载体以大小,药物释放,毒性进行了表征,并使用各种测定方法评估了它们对*P. aeruginosa*菌株和临床分离物的抗菌和抗菌膜活性,包括共焦激光扫描显微镜 (CLSM).
主要成果:
- 开发的SLN表现出长时间的QSI释放,并且对肺细胞没有急性毒性 (A549,Calu-3).
- 酸盐涂层增强了SLN吸收,而酸盐酶固定显著增加了SLN扩散和生物膜透.
- 阴性SLN有效地抑制了生物膜的形成并根除了已建立的生物膜,而阴性SLN意外地促进了生物膜的生长. 酸酶固定增强了生物膜的脆弱性,但在某些情况下,酸涂层增加了生物膜的形成.
- SLN化增加了生物膜密度,在P. aeruginosa*生物膜中观察到显著的吸收,特别是非粘膜菌株中CS涂层的SLN.
结论:
- 开发的QSI/SLNs/AL系统显示出显著的生物膜破坏性潜力,为囊性纤维化病的特定部位,生物膜向性干预治疗提供了一个有希望的策略.
- 进一步研究控制纳米载体-生物膜相互作用的复杂内在和外在因素对于优化治疗疗效至关重要.
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