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在切片和厚度测量过程中持续冷却样本,有助于提高病理样本图像分析的准确性
Masanao Saio1, Raia Kushibiki2, Yuki Kanehira2
1Laboratory of Histopathology and Cytopathology, Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, Gunma, Japan. saio@gunma-u.ac.jp.
Histology and histopathology
|May 6, 2024
概括
连续冷却装置 (CCD) 提高了组织样本的断面厚度 (ST) 均性. 一致的ST对于在组织病理学中进行准确的定量分析至关重要,确保可靠的染色结果.
科学领域:
- 组织病理学 组织病理学
- 生物医学成像技术 生物医学成像技术
- 材料科学 材料科学 材料科学
背景情况:
- 在组织病理学中,准确的定量分析依赖于一致的样本截面厚度 (ST).
- 在ST的变化可以显著影响的正染色的百分比面积,影响诊断和研究结果.
研究的目的:
- 为了研究断面厚度 (ST) 与正色彩的百分比面积之间的关系.
- 评估冷却方法 (冰包与连续冷却装置) 对ST统一性的影响.
主要方法:
- 从仅含和FFPE块使用冰袋 (IP) 或连续冷却装置 (CCD) 准备了截面.
- 用薄膜厚度计测量了截面厚度.
- 染色是使用欧或氨酸蓝色进行的.
- 计算机辅助图像分析量化了阳性染色的百分比面积及其与ST的相关性.
主要成果:
- 与冰箱 (IP) 相比,连续冷却装置 (CCD) 产生了明显更薄,更均的截面.
- 在ST和欧和氨酸蓝的阳性染色的百分比面积之间发现了显著的相关性.
- 与具有变异性ST的部分相比,具有均ST的部分显示出明显不同的染色区域.
结论:
- 建议使用连续冷却装置 (CCD) 进行样本切割,以实现统一的ST.
- 在染色前测量ST对于准确的定量组织病理学分析至关重要.
- 组织学检查应使用具有统一ST的标本,以获得可靠的结果.
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