改变B细胞表位的竞争环境可以显著延长抗体生产的持续时间
Hongke Xu1, Yanfei Chen2, Jingzhi Li1
1Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
International immunology
|May 6, 2024
概括
通过修改抗原呈现来实现持续性免疫球蛋白G (IgG) 生产 (PIP). 使用牛血清白蛋白合物将短暂的IgG产生 (TIP) 转化为长期的疫苗保护.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
- 生物化学 生物化学
背景情况:
- 持续的免疫球蛋白G (IgG) 生产 (PIP) 对于疫苗的长期疗效至关重要.
- 影响PIP持续时间的因素,特别是在基于的疫苗中,仍然不太清楚.
- 了解这些因素对于开发更有效,更持久的疫苗至关重要.
研究的目的:
- 研究氨基酸序列,蛋白质载体和免疫策略如何影响抗IgG生产的持续时间.
- 为了确定短暂的IgG产量 (TIP) 是否可以转换为PIP.
- 探索开发新型疫苗的潜力.
主要方法:
- 羊被免疫小反动物瘟疫 (PPR) 活衰减疫苗和/或牛血清白蛋白 (BSA) - 联体.
- 随着时间的推移,对抗IgG水平进行了监测,以评估抗体生产的持续时间 (TIP与PIP).
- 评估了不同免疫计划和抗原呈现方法 (病毒颗粒与BSA合物) 的影响.
主要成果:
- 当是PPR疫苗的一部分时,抗IgG的产生是暂时的 (TIP),但当通过BSA结合物呈现时是持久的 (PIP).
- 由BSA-联体诱导的PIP持续时间超过368天,并且没有受到随后的PPR疫苗免疫接种的影响.
- 与PPR疫苗和BSA-联体同时接种导致TIP的适度延长,约为100天.
结论:
- 抗原的呈现环境显著影响IgG生产的持续时间,允许从TIP转换为PIP.
- 这一发现为血细胞命运和针对线性表位体的耐用类疫苗的开发提供了新的见解.
- 使用像BSA这样的蛋白质载体的策略可以提高疫苗应用中的抗体反应的寿命.
相关概念视频
01:24B Cell Activation and Differentiation
1.7K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.7K
01:31Hybridoma Technology
14.7K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
14.7K
01:41Affinity and Avidity
36.0K
Overview
36.0K
00:42Cross-reactivity
31.0K
Overview
31.0K
01:36Humoral Immune Responses
72.6K
Overview
72.6K
01:28Diversity of Antigen Receptors
574
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
574