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血衍生的细胞外囊泡可以改善小鼠胚胎发育.

Maryam Javadi1,2, Mohammad Sadegh Gholami Farashah3,4, Leila Roshangar3,5

  • 1Department of Anatomical Sciences, Faculty of Medicine, Zanjan University of Medical Sciences (ZUMS), Zanjan, Iran. maryamjavadi22@yahoo.com.

Molecular biology reports
|May 6, 2024
PubMed
概括

血衍生的细胞外囊泡 (EVs) 增强了小鼠胚胎的发育. 用这些EV补充体外成熟介质显著改善了受精率和早期胚胎发育率.

关键词:
电子显微镜电子显微镜在受精过程中,受精卵细胞成熟的过程由等离子体衍生的电动汽车.

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科学领域:

  • 生殖生物学 生殖生物学
  • 细胞生物学 细胞生物学
  • 细胞外囊泡研究研究

背景情况:

  • 研究血衍生细胞外囊泡 (EVs) 对哺乳动物生殖过程的影响.
  • 对比EV与传统介质在支持早期胚胎发育方面的疗效.

研究的目的:

  • 确定血衍生细胞外囊泡 (EVs) 对小鼠受精和早期胚胎发育的影响.
  • 评估电动车作为辅助生殖技术的补充品的潜力.

主要方法:

  • 通过超离心法从小鼠血中分离细胞外囊泡 (EVs).
  • 使用动态光散射 (DLS) 和传输电子显微镜 (TEM) 进行电动汽车的表征.
  • 西方涂抹用于识别EV蛋白标记物 (例如CD82,HSP90) 并评估它们对生殖蛋白的影响.

主要成果:

  • 血衍生的EVs对小鼠的受精率产生了积极影响 (P<0.05).
  • 用EVs补充剂显著增加了早期胚胎发育率,包括8细胞,毛囊和胚囊阶段 (P < 0.01).
  • EVs成功地被纳入卵细胞细胞质中,如DiI标记的EVs所示.

结论:

  • 在体外成熟介质中补充血衍生的EVs对小鼠胚胎发育有好处.
  • 电动化器作为改善体外受精和胚胎培养成功率的新型组件具有前景.