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针对pentamidine针对CD44过度表达细胞使用化脂聚合物混合纳米颗粒

Ilaria Andreana¹, Marta Chiapasco¹, Valeria Bincoletto¹

¹Dipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Torino, Italy.

Drug delivery and translational research

|May 6, 2024

概括

研究人员开发了新型混合纳米载体,使用聚乳糖合糖酸 (PLGA) 和氨酸 (HA) 进行向药物输送. 这些纳米颗粒显示了增强的药物负载,并选择性地向过度表达CD44受体的癌细胞.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
药物输送系统药物输送系统

背景情况:

可生物降解的纳米载体为控制和向的药物释放提供了潜力.
脂质体和聚合物纳米粒子是已建立的药物递送系统.
混合纳米载体可以结合不同平台的优势,以提高效率和定位.

研究的目的:

为了制定混合纳米载体,将聚乳-co-糖酸 (PLGA) 和氨酸 (HA) 结合起来,用于主动向.
为了利用离子相互作用来增强药物封装,使用胺胺 (PTM-B) 作为模型药物.
评估混合纳米载体对癌细胞的向传递和细胞毒性.

主要方法:

用氨酸-脂联合体 (HA-DPPE) 配制PLGA纳米颗粒,用于HA的表面固.
通过负电荷的PLGA/HA和正电荷的胺素之间的离子配对来进行药物加载.
纳米载体大小,泽塔潜力,内部结构和封装效率的表征.
细胞吸收和细胞毒性对CD44过度表达的癌细胞的体外评估.

主要成果:

成功制备了大小小小于150nm的混合纳米载体,zeta潜力为-35mV.
通过离子配对实现了胺胺的高封装效率 (90%).
在过度表达CD44.4的癌细胞上证明了优选的细胞吸收和受体介导的细胞毒性.
确认了纳米颗粒的CD44依赖细胞内化.

结论:

开发的PLGA/HA-DPPE混合纳米载体代表了针对药物输送的有希望的平台.
与传统的PLGA纳米粒子相比,离子配对方法提高了药物载荷能力.
CD44介导的向机制可以选择性地传递给癌细胞,从而有可能改善治疗结果.

关键词:

氨酸是什么? 氨酸是什么?脂质聚合物混合纳米颗粒在 PLGA PLGA 中.五胺氨酸 (Pentamidine) 是一种可以脂联合酸盐的结合物

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