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Updated: Jun 27, 2025

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
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PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

Published on: July 2, 2010

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一种双硫酸盐辅助和基于结合的qPCR放大技术,用于在基底分辨率下特定位置的伪乌里丁检测

Xin Fang1, Ruiqi Zhao2, Yafen Wang3

  • 1College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers-Ministry of Education, Wuhan University, Wuhan, Hubei 430072, P. R. China.

Nucleic acids research
|May 6, 2024
PubMed
概括

一种新的方法,伪尿素-TRACE (pseU-TRACE),准确地检测RNA中的伪尿素 (Ψ) 修饰在基底分辨率. 这种敏感的技术有助于理解 Ψ Ψ .

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Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR08:02

Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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