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ZmASR1对玉米的干旱应激耐受性进行负面调节

Yun Yang¹, Aiqi Li¹, Yuqing Liu¹

¹National Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei, Anhui, 230036, China.

Plant physiology and biochemistry : PPB

|May 6, 2024

概括

这项研究表明,玉米中的ZmASR1基因对干旱耐受性产生负面影响. 淘汰植物通过减少反应性氧物种和增强酸信号传递来增加耐药性.

科学领域:

植物分子生物学植物分子生物学
无生物应激反应的应激反应
玉米的遗传学玉米的遗传学

背景情况:

亚酸,压力和成熟诱导 (ASR) 蛋白质对于植物应激反应至关重要.
玉米中ASR基因的特定功能,特别是干旱,尚不清楚.

研究的目的:

鉴定和功能性描述玉米中一种新的干旱诱导的ASR基因,称为ZmASR1.1.
阐明ZmASR1在玉米干旱耐受性中介作用.

主要方法:

在干旱和酸 (ABA) 处理下识别和描述ZmASR1基因表达.
使用CRISPR-Cas9技术生成ZmASR1的淘汰线.
在野生类型和淘汰植物中分析生理参数 (ROS,ABA含量,口腔关闭) 和基因表达 (转录组测序).

主要成果:

干旱压力和ABA诱导ZmASR1转录,蛋白质定位在细胞核和细胞质中.
与野生类型相比,ZmASR1淘汰线表现出减少反应性氧物种 (ROS) 积累,增加ABA含量和增强的口腔关闭.
转录组分析显示,在对干旱的反应中,ABA信号,抗氧化剂防御和光合作用途径的丰富.

结论:

ZmASR1对玉米的干旱耐受性进行负面调节.
ZmASR1通过调节ABA信号,ROS积累和口腔功能来起作用.
ZmASR1是基因工程的潜在候选基因,用于改善玉米的抗干旱能力.

关键词:

在 ABA 信号传输中.干旱造成的压力是干旱.有反应性氧物种的反应性氧物种.这就是ZmASR1的意思.

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