氧化脱系统的微生态结构是由硫甲醇的循环应力驱动的
Xiao-Yan Fan1, Zhong-Xing Zhang2, Xing Li1
1Faculty of Architecture, Civil and Transportation Engineering, Beijing University of Technology, Beijing 100124, PR China.
Bioresource technology
|May 6, 2024
概括
这项研究开发了有氧脱化 (AD) 系统,能够抵抗抗生素压力. 循环硫甲醇 (SMX) 暴露提高了去除效率达95%,证明了废水处理的可行策略.
科学领域:
- 环境微生物学 环境微生物学
- 废水处理工程 废水处理工程
- 抗生素耐药性 抗生素耐药性
背景情况:
- 有氧脱 (AD) 系统在抗生素压力环境中面临挑战.
- 硫甲醇 (SMX) 是一种常见的抗生素污染物,影响微生物群落.
- 在抗生素压力下开发强大的AD过程对于有效的废水处理至关重要.
研究的目的:
- 调查循环应激策略,使用硫甲醇 (SMX) 度梯度,以使有氧脱 (AD) 在测序批量反应器 (SBR) 中.
- 评估SMX压力对与AD相关的微生物社区结构和功能的影响.
- 阐明机制,包括抗生素耐药性基因 (ARG) 和代谢途径,支持在抗生素压力下AD.
主要方法:
- 实施一个测序批量反应器 (SBR) 的循环度梯度 (5-30 mg/L) 的硫甲醇 (SMX).
- 监测总去除效率的情况.
- 微生物群落组成的分析,重点是丰富-罕见的属和条件罕见或丰富的种类 (CRAT).
- 研究用于有氧脱的关键功能基因 (AmoABC,napA,nirK) 和抗生素耐药性基因 (ARGs) 的共同表达.
主要成果:
- 在周期性SMX压力下,总去除效率从大约10%大幅增加到95%.
- SMX压力改变了微生物属的反应,特别是影响了条件罕见或丰富的种群 (CRAT).
- 针对AD的功能基因 (AmoABC,napA,nirK) 与ARG (acrR,ereAB,mdtO) 共同表达,表明了协同适应.
- 抗生素耐药性基因 (ARG) 和三碳酸 (TCA) 循环增强了抗氧化剂和电子运输能力,这对AD至关重要.
结论:
- 循环SMX应力是SBR中建立功能性有氧脱 (AD) 的有效策略.
- 抗生素外流所促进的AD功能基因和ARG之间的协同相互作用是系统弹性的关键.
- 这项研究支持调节活性污泥在现场AD功能,即使存在抗生素污染.
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