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三叶草因子3可以刺激Th17细胞响应在2型糖尿病的发展中

Ziyang Lin¹, Jinyuan Zhang¹, Tingting Duan²

¹Key Laboratory of Glucolipid Metabolic Diseases of the Ministry of Education, Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine, The Institute of Chinese Medicinal Sciences, Science and Technology Building, Guangzhou Higher Education Mega Centre, Guangdong Pharmaceutical University, 280 Wai Huan Dong Road, Guangzhou, 510006, People's Republic of China.

Scientific reports

|May 6, 2024

概括

三叶草因子3 (TFF3) 可以促进2型糖尿病 (T2DM) 的炎症. 在T2DM模型中,减少TFF3可以改善葡萄糖利用率和胰腺健康.

科学领域:

免疫学免疫学免疫学
内分泌学在内分泌学.
分子生物学分子生物学

背景情况:

三叶草因子3 (TFF3) 都涉及到各种生物过程.
在2型糖尿病 (T2DM) 的炎症方面,TFF3的作用仍未得到充分研究.
在T2DM的发病过程中,T细胞的功能至关重要.

研究的目的:

研究TFF3在T2DM中的作用,重点关注其对T细胞功能和炎症的影响.
阐明TFF3影响T2DM相关免疫反应的潜在机制.

主要方法:

使用lentivirus在Jurkat T细胞中构建了一个TFF3过度表达模型.
在高葡萄糖条件下评估TFF3对Jurkat细胞增殖,细胞亡和IL-17A水平的影响.
诱导T2DM在TFF3淘汰赛 (KO) 和野生类型小鼠中使用链毒素和高脂肪饮食.
评估了小鼠的葡萄糖/胰岛素耐受性,炎症标志物,Th17细胞比例和胰腺病理.

主要成果:

在Jurkat细胞中TFF3过度表达增加了IL-17A+ T细胞和Th17相关的细胞因子.
在小鼠中的T2DM建模增加了脏TFF3的表达,并引起了腹巨变.
与野生型T2DM小鼠相比,TFF3KO小鼠表现出改善的葡萄糖利用率,减少的胰腺病理,较低的炎症和较少的Th17细胞.
野生型和TFF3 KO小鼠之间没有观察到体重或血糖水平的显著差异.

结论:

TFF3在与T2DM相关的慢性炎症性免疫反应中发挥作用.
通过调节RORγt/IL-17信号通路,TFF3可以发挥其作用.
TFF3影响T细胞增殖和细胞亡,有助于T2DM的发病.

关键词:

辅助型T淋巴细胞 17 炎症炎症是一种炎症.三叶树的三叶子因素3 2型糖尿病是什么? 2型糖尿病是什么?

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