与非吸烟者相比,香烟吸烟者和无烟烟草使用者之间的口腔微生物组失调
Suhana Chattopadhyay1, Leena Malayil1, Jessica Chopyk1
1Department of Global, Environmental, and Occupational Health, School of Public Health, University of Maryland, College Park, MD, USA.
Scientific reports
|May 6, 2024
概括
吸烟会改变口腔微生物群,增加细菌的多样性. 在四个月的时间里,与非吸烟者相比,在吸烟者和无烟者中观察到特定的细菌转移.
科学领域:
- 微生物学 微生物学
- 口腔健康 口腔健康
- 人类微生物组 人类微生物组
背景情况:
- 吸烟是影响口腔健康的一个重要因素.
- 不同的烟草制品对口腔微生物群的长期影响仍未得到充分研究.
研究的目的:
- 在四个月的时间内,研究和比较吸烟者,无烟烟草使用者和非使用者的口腔微生物组组成.
- 确定与不同形式的烟草消费相关的时间变化和特定的细菌种群.
主要方法:
- 纵向研究设计,采用四个采样时间点,持续四个月.
- 从85名参与者 (吸烟者,无烟烟草使用者,非使用者) 采集口腔拭子和唾液样本.
- 在Illumina MiSeq上进行16S rRNA基因测序 (V3-V4区域),用于微生物社区分析.
主要成果:
- 与非吸烟者相比,烟草使用者表现出更大的口腔细菌多样性.
- 在烟草使用者中观察到更高的Firmicutes的相对丰度和更低的蛋白质细菌的丰度.
- 在烟草使用者和非使用者之间,在像Actinomyces,Neisseria和Prevotella这样的属的相对丰度上发现了明显的差异.
- 在为期四个月的研究期间,一些细菌物种的丰富性呈现出显著的变化.
- 在烟草使用者身上发现了机会性病原体,如Neisseria subflava,Bulleidia moorei和Porphyromonas endodontalis.
结论:
- 不同的烟草产品对口腔微生物群的结构和多样性产生不同的影响.
- 纵向分析揭示了烟草使用者的口腔内特定细菌物种的时间动态.
- 这些发现有助于全面了解烟草诱导的口腔失生症以及机会性病原体的潜在作用.
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