在淋巴细胞形中,细胞内网膜压力通过糖溶解减弱
Xuecong Li1,2, Wenqun Zhong1,2, Hao Jiang1,2
1State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
Pediatric research
|May 6, 2024
概括
糖溶解有助于人类皮肤淋巴内皮细胞抵抗内 плазма网膜 (ER) 压力,这是淋巴形 (LM) 发育的关键过程. 激活ER压力可能为LM提供治疗策略.
科学领域:
- 血管生物学 血管生物学
- 细胞应激反应的应激反应
- 代谢调节 代谢调节 代谢调节
背景情况:
- 细胞内膜网膜 (ER) 压力在人类皮肤淋巴内皮细胞 (HDLEC) 和淋巴形 (LMs) 中起作用.
- 在这些细胞中,ER压力,有氧糖解和炎症之间的相互作用尚未完全理解.
研究的目的:
- 研究ER应激在HDLEC和LM中的作用.
- 在LMS的背景下探索ER压力,有氧糖解和炎症之间的关系.
主要方法:
- 在接受脂多糖 (LPS) 治疗后,评估了HDLEC增殖和亡.
- 用西方斑点,海马分析和乳酸测定来量化ER压力和糖解标记.
- 免疫组织化学被用来评估LM组织中的ER压力.
主要成果:
- 在HDLEC中,LPS诱导了ER压力,但没有引起显著的亡,同时增加了糖解.
- 敲除赫索金酶2降低了细胞抵抗ER压力诱导的亡的能力.
- 在LM中,ER压力标志物 (GRP78/BIP,p-PERK) 升高调节,与赫索金酶2表达相关.
- 在大鼠模型中,药理ER应激激活抑制了LM的进展.
结论:
- 葡萄糖溶解可以减轻HDLEC中ER压力诱导的亡,支持LM的发展.
- 在LM中,ER压力被激活,特别是在炎症条件下.
- 对于治疗LMs来说,ER压力是一个潜在的治疗目标.
相关概念视频
Role of ER in the Secretory Pathway
5.4K
Eukaryotic cells have a special pathway that enables communication between various intracellular membrane-bound compartments and also with the extracellular environment. This pathway is termed as the secretory pathway.
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
5.4K
Smooth Endoplasmic Reticulum
5.7K
Smooth endoplasmic reticulum or smooth ER is a sub-organelle with specialized functions in animal cells and plant cells. It is often associated with the tubule morphology of the endoplasmic reticulum.
The ER provides optimal conditions for synthesizing steroid hormones and lipids, such as phospholipids and triglycerides. Traditionally, lipid metabolism was considered to be a smooth ER function. However, there is no direct evidence to prove that rough ER is completely excluded from lipid...
The ER provides optimal conditions for synthesizing steroid hormones and lipids, such as phospholipids and triglycerides. Traditionally, lipid metabolism was considered to be a smooth ER function. However, there is no direct evidence to prove that rough ER is completely excluded from lipid...
5.7K
The Endoplasmic Reticulum
13.3K
The endoplasmic reticulum or ER makes up for more than half of the membranes in a cell and accounts for 10% of total cell volume. It is also the primary protein and lipid synthesis factory for most cell organelles, such as the Golgi apparatus, lysosomes, secretory vesicles, and the plasma membrane. Despite being the most extensive and functionally complex subcellular organelle, ER was the last to be discovered. After years of deliberation, Keith Porter and George Palade in the year 1954,...
13.3K
Lysosomal Hydrolases
3.8K
Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
3.8K
What is Glycolysis?
164.6K
Overview
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...
164.6K


