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切断结合体"绳子蛋白"Prp45的结果是依赖Htz1的表型

Kateřina Abrhámová¹, Martina Groušlová¹, Anna Valentová¹

¹Department of Cell Biology, Faculty of Science, Charles University, Praha, Czech Republic.

|May 6, 2024

概括

酵母拼接因子 Prp45 与染色质修饰剂如 H2A.Z. 相互作用. 这项研究揭示了Prp45如何使用Prp45.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
染色体生物学染色体生物学

背景情况:

剪切细胞组合对于基因表达调节至关重要.
Prp45是一种酵母拼接因子,参与拼接酶组合.
Prp45在将结合体组件组合在一起方面的精确作用尚未完全理解.

研究的目的:

为了研究 Prp45 断的等位基因 (prp45~1-169) 的遗传相互作用,以了解其功能.
探索结合体功能和染色体调节之间的关系.
为了确定内部去除对与Prp45切断相关的基因表达表型的影响.

主要方法:

使用合成遗传阵列技术进行全基因组遗传相互作用分析.
用修改的PRP45等位基因和内无基因构建和分析酵母菌株.
评估生长表型和mRNA前积累.

主要成果:

染色体重塑剂和修饰剂,包括H2A.Z (HTZ1) 和SWR1,INO80,SAGA复合体,被确定为prp45的显著遗传相互作用剂 ((1-169).
删除HTZ1导致了最严重的生长缺陷.
从SRB2中删除了内部基因,但没有其他测试的基因,部分挽救了生长表型.
prp45和htz1之间的合成遗传相互作用即使在SRB2内核缺失的情况下也持续存在,并且在低温下观察到较少截断的Prp45变体.
htz1删除在prp45(1-330) 突变者中加剧了前mRNA积累.

结论:

prp45 ((1-169) 的基因互动组受到低副本内核含量基因表达缺陷的影响.
Prp45和Htz1之间的遗传相互作用突出显示了结合体组合对染色质环境的敏感性.
Prp45的功能与染色体调节有关,影响拼接效率和基因表达.

关键词:

在H2A.Z.合成遗传阵列分析合成遗传阵列分析染色体修饰剂染色体修饰剂同转录的拼接.结合体组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合

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