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T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

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Updated: Jun 27, 2025

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

Published on: July 30, 2015

解读TET3互动组在初级胸膜发育T细胞中的解密.

Dimitris Theofilatos¹, Tricia Ho², Greg Waitt²

¹Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

|May 7, 2024

概括

十一转位3 (TET3) 蛋白与T细胞发育中的众多合作伙伴相互作用. 这些相互作用揭示了TET3.

关键词:

生物化学生化学分子生物学分子生物学分子相互作用分子相互作用.蛋白质组学是指蛋白质组学.

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Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

Published on: August 8, 2014

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Last Updated: Jun 27, 2025

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Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

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科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

十一转位 (TET) 蛋白质是DNA二氧化酶,对于活性DNA脱甲基化至关重要.
在发育中的T细胞中,TET3的表达很高,并与T细胞系谱规范有关.
缺乏TET的小鼠表现出复杂的表型,这表明TET3除了脱甲基化之外还有多方面的作用.

研究的目的:

使用体内条件在初级发育的T细胞中识别内源性TET3相互作用伙伴.
通过其蛋白互动组来阐明TET3的更广泛的生物功能.

主要方法:

使用双重质谱 (LC-MS/MS) 的液体染色学.
对初级发育的T细胞进行了分析,以捕捉内源性相互作用.

主要成果:

确定了TET3互动合作伙伴的全面列表.
发现TET3参与各种细胞过程,包括转录调节,RNA聚合酶延长,拼接,DNA修复和DNA复制.

结论:

TET3参与了广泛的基本生物过程,超出了其在DNA脱甲基化中的已知作用.
鉴定到的互动体为未来研究TET3在T细胞生物学中的精确机制提供了基础.