相关实验视频

Updated: Jun 27, 2025

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

滴滴Hi-C为单细胞中染色体结构的快速和可扩展的配置文件

Lei Chang¹, Yang Xie^1,2, Brett Taylor^1,3

¹Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA, USA.

bioRxiv : the preprint server for biology

|May 7, 2024

概括

滴滴Hi-C能够在复杂组织中进行高通量,单细胞染色质构成分析. 这种多功能技术可以绘制基因调节的地图,并检测正常和患病细胞中的基因组变异.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
生物技术是生物技术.

背景情况:

了解染色体结构对于发育和疾病中的基因调节至关重要.
现有的方法在异质组织样本方面存在困难.
需要单细胞分辨率才能进行准确的分析.

结论:

滴滴Hi-C克服了分析异质组织的局限性.
这种技术为探索健康和疾病中的基因调节提供了多功能工具.
促进全面了解染色体结构在基因表达和疾病发病过程中的作用.

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Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

Capturing Chromosome Conformation Across Length Scales

Capturing Chromosome Conformation Across Length Scales

Published on: January 20, 2023

相关概念视频

Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...