PARP14是亲病毒和抗病毒宿主因子,促进IFN的产生,并影响多种病毒的复制
Srivatsan Parthasarathy1, Pradtahna Saenjamsai1, Hongping Hao1
1Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045, USA.
bioRxiv : the preprint server for biology
|May 7, 2024
概括
聚 ((ADP-ribose) 聚合酶14 (PARP14) 增强干扰素的产生和限制病毒复制,表现出对冠状病毒和简单疹病毒等多种病毒的亲和抗病毒功能.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- 聚ADP-ribose) 聚合酶14 (PARP14) 以其在DNA损伤,炎症和癌症中的作用而闻名.
- PARP14是由干扰素 (IFN) 上调调节的,并且涉及宿主-病原体相互作用.
- 以前的研究表明,PARP14在冠状病毒感染期间参与IFN-I的产生,但其直接的抗病毒作用尚不清楚.
研究的目的:
- 研究PARP14的直接抗病毒功能.
- 为了确定PARP14的催化活性是否对其抗病毒作用至关重要.
- 评估PARP14对冠状病毒以外的广泛病毒的影响.
主要方法:
- 利用PARP14淘汰赛 (KO) A549细胞研究病毒复制.
- 感染了各种病毒的KO细胞,包括小鼠肝炎病毒 (MHV),SARS-CoV-2,简单疹病毒1 (HSV-1),囊性口腔炎病毒 (VSV),埃博拉病毒 (EBOV) 和尼帕病毒 (NiV).
- 用PARP14活性部位抑制剂来评估催化活性的作用.
主要成果:
- PARP14的催化活性增强了IFN-I和IFN-III的反应.
- PARP14限制了ARH缺乏MHV和SARS-CoV-2的复制.
- PARP14限制了HSV-1复制,但对于VSV,EBOV和NiV的有效感染至关重要.
- PARP14对HSV-1和EBOV的抗病毒作用独立于其催化活性.
结论:
- PARP14促进干扰素的产生,有助于抗病毒防御.
- PARP14表现出上下文依赖的抗病毒功能,限制一些病毒,同时促进其他病毒.
- PARP14在宿主对病毒感染的反应中发挥着多方面的作用,影响多个病毒家族.
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