双边细胞流在胚胎胃化中,在左右组织者形成之前显示不对称
Rieko Asai1,2, Shubham Sinha3, Vivek N Prakash3,4,5
1Cardiovascular Research Institute, University of California, San Francisco. San Francisco, California 94158, USA.
bioRxiv : the preprint server for biology
|May 7, 2024
概括
早期胚胎发育在中线形成之前显示左右不对称. 反旋转的细胞运动,或反旋转的细胞运动.
科学领域:
- 发育生物学 发展生物学
- 生物物理学的生物物理.
- 胚胎学 胚胎学
背景情况:
- 双边的身体计划对动物发展至关重要.
- 胚胎胚胎的左右不对称模式是在胃流动过程中建立的.
- 早期左右不对称的时间和机制,先于中线形态发生,尚未完全理解.
研究的目的:
- 为了研究早期羊水胚胎胃流动期间的左右不对称模式.
- 探索细胞运动在建立胚胎左右不对称性中的作用.
- 为了确定左-右不对称性是否先于关键中线结构的形成.
主要方法:
- 利用小胚胎模型系统进行高空间和时间分辨率的生物物理量化.
- 分析了大规模的双边反旋转细胞流,称为"波罗奈斯运动".
- 量化细胞流速和旋转,以确定不对称的时间和位置.
- 研究了线粒体停止对细胞流动模式和不对称性的影响.
主要成果:
- 在早期胃流动期间,在原始条纹和亨森结节形成之前,检测到"波罗奈斯运动"的左右不对称.
- 在细胞运动开始后6小时开始的双边流中观察到一致的右侧主导地位.
- 线性逮捕改变了流动模式,但并没有废除右派的统治地位,这表明早期的物理影响.
结论:
- 胎儿胃口的左右不对称性可以比目前的模型更早地检测到.
- 物理过程,如细胞流动,在建立胚胎左右横向性方面发挥着重要作用.
- 这些发现挑战了假设不对称性仅仅来自节点的横向性基因调节的模型.
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