深度学习驱动的细胞分裂和细胞生长在整个真核生物生命周期的成像
Shreya Ramakanth1, Taylor Kennedy1, Berk Yalcinkaya1
1Department of Plant and Microbial Biology, North Carolina State University.
bioRxiv : the preprint server for biology
|May 7, 2024
概括
研究人员开发了一种深度学习成像框架,通过其完整生命周期量化跟踪真核微生物. 这种方法揭示了细胞生长之前从非增殖状态的退出,提供了对微生物生命周期调节的新见解.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 生物成像是一种生物成像.
背景情况:
- 细胞微生物的生命周期涉及繁殖和非繁殖状态之间的复杂过渡.
- 了解这些转变对于开发针对性药物,杀虫剂和针对病原体的疫苗至关重要.
- 目前的方法通常依赖于部分观测的理论组合,缺乏定量严谨性.
研究的目的:
- 开发一个定量,深度学习驱动的成像框架,用于跟踪完整的真核微生物生命周期.
- 在Saccharomyces cerevisiae的性生命周期期间分析细胞生长和细胞周期调节.
- 为描述不完全描述的单细胞真核生物生命周期提供一种工具.
主要方法:
- 结合微流体培养与基于深度学习的图像细分 (卷积神经网络).
- 开发了一种使用深度学习视频插值的新型细胞跟踪算法 (FIEST).
- 使用了Whi5蛋白 (酵母Rb模拟) 的光报告器和一个高Cdk1活性传感器 (LiCHI).
主要成果:
- 观察到细胞生长先于从非增殖状态 (G1,G0) 退出.
- 减少Whi5的总细胞度标志着从非增殖状态的退出.
- 细胞分裂和生长的时间协调在三个性世代保持一致.
结论:
- 开发的深度学习框架可以进行定量成像和追踪真核生物生命周期.
- 细胞生长动态和Whi5调节为细胞周期控制在增殖退出期间提供了新的见解.
- 酵母视觉界面为微生物生命周期研究提供了可访问的工具.
相关概念视频
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00:56What is the Cell Cycle?
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