相关实验视频

Updated: Jun 27, 2025

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

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MOSAIC使基因的in situ和突变发生和CRISPR主要编辑指南RNA优化在人类细胞中成为可能

Jonathan Y Hsu, Kin Chung Lam, Justine Shih

    bioRxiv : the preprint server for biology
    |May 7, 2024
    PubMed
    概括

    在现场特征化 (MOSAIC) 方法的特定变化的多重复合使得使用CRISPR主要编辑快速,集成安装数千个基因编辑. 这种强大的工具加速了基因功能和耐药性研究的高通量查.

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    Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing09:03

    Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

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    Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli07:04

    Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

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    相关概念视频

    CRISPR01:59

    CRISPR

    Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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    Homologous Recombination02:31

    Homologous Recombination

    The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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