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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Updated: Jun 27, 2025

Quantitative FRET Förster Resonance Energy Transfer Analysis for SENP1 Protease Kinetics Determination

Quantitative FRET Förster Resonance Energy Transfer Analysis for SENP1 Protease Kinetics Determination

Published on: February 21, 2013

通过单分子显微镜测量PETase酶动态.

Yuwei Zhang¹, William O Hancock^1,2

¹Department of Biomedical Engineering, Pennsylvania State University, University Park, Pennsylvania, USA.

bioRxiv : the preprint server for biology

|May 7, 2024

概括

一种新的单分子测定追踪了PETase酶降解塑料的过程. 这种方法揭示了酶如何与聚乙烯二甲 (PET) 结合和相互作用,有助于开发更好的塑料降解酶.

科学领域:

生物化学生物化学
环境科学环境科学
材料科学材料科学材料科学

背景情况:

聚乙烯二甲 (PET) 是微塑料污染的主要来源.
对于环境修复而言,PET的微生物和酶降解至关重要.
传统的酶动力学测试对PET等不溶性基质具有挑战性.

结论:

单分子测试克服了不溶性基质的批量测试的局限性.
这种方法有助于解释酶活性,并指导蛋白质工程.
增强的PETase酶对于打击微塑料污染至关重要.

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Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Published on: July 25, 2012

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Last Updated: Jun 27, 2025

Quantitative FRET Förster Resonance Energy Transfer Analysis for SENP1 Protease Kinetics Determination

Quantitative FRET Förster Resonance Energy Transfer Analysis for SENP1 Protease Kinetics Determination

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Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

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Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

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