Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

An Integrated muti-omics cell atlas of the human trabecular meshwork and ciliary body.

bioRxiv : the preprint server for biology·2026
Same author

Transcriptomic Atlas of Human Trabecular Meshwork Uncovers the Cellular Landscape and Provides Insights into Glaucoma Pathophysiology.

Research square·2026
Same author

Potential of extracellular vesicle-derived microRNAs as a platform for biomarker discovery in acute lymphoblastic leukemia.

PloS one·2026
Same author

Transient YAP activation uncovers the neurogenic potential of proliferative mammalian Müller glia.

PNAS nexus·2026
Same author

High KIR diversity in Uganda and Botswana children living with HIV.

Human immunology·2026
Same author

Expanding the Mutation Spectrum of Non-Syndromic Retinitis Pigmentosa in Consanguineous Pakistani Families: Unraveling Novel Pathogenic Variants in <i>RP1, PDE6B</i>, and <i>PRCD</i> Genes for Precision Diagnosis.

Genes·2026

相关实验视频

Updated: Jun 27, 2025

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

3.6K

样本多重复合用于视网膜单细胞RNA测序.

Justin Ma, Ting Kuan Chu, Maria Polo Prieto

    bioRxiv : the preprint server for biology
    |May 7, 2024
    PubMed
    概括

    样本复杂化可提高罕见细胞群的单细胞RNA测序 (scRNA-seq) 精度. 这种方法用独特的条形码标记样本,使得能够对实验中的细胞类型和变异进行准确的分析.

    科学领域:

    • 基因组学就是基因组学.
    • 分子生物学分子生物学
    • 神经科学是一个神经科学.

    背景情况:

    • 用微流体单细胞RNA测序 (scRNA-seq) 鉴定罕见细胞群的特征是具有挑战性的.
    • 当前的丰富和聚合方法掩盖了样品来源和表型数据,特别是在高变异实验中.

    研究的目的:

    • 为scRNA-seq.开发和实施一个样本复合管道.
    • 为了提高分析小鼠视网膜质细胞细胞类型分布和转录组变异的精度.

    主要方法:

    • 建立了一个scRNA-seq样本复合管道,使用胆固醇修饰的寡头.
    • 为了提高精度,将管道应用于小鼠视网膜质细胞.

    主要成果:

    • 成功实施了对小鼠视网膜质细胞的样本复合.
    • 在调查细胞类型分布和转录组变异方面取得了更高的精度.

    结论:

    • 样本复杂化是提高scRNA-seq分析精度的一个有价值的方法.
    • 这种技术对于涉及罕见细胞种群,疾病模型和人体样本的研究特别有用.

    更多相关视频

    Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
    08:30

    Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

    Published on: January 7, 2020

    13.0K
    Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
    11:26

    Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

    Published on: May 22, 2017

    13.8K

    相关实验视频

    Last Updated: Jun 27, 2025

    Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
    06:24

    Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

    Published on: March 12, 2021

    3.6K
    Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
    08:30

    Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

    Published on: January 7, 2020

    13.0K
    Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
    11:26

    Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

    Published on: May 22, 2017

    13.8K