高血糖症通过其对内皮细胞特征和功能影响而扰乱血脑屏障的完整性
Arshad Hashmat1, Jingyuan Ya1, Rais Kadir1
1Academic Unit of Mental Health and Clinical Neurosciences, School of Medicine, The University of Nottingham, Nottingham, UK.
Tissue barriers
|May 7, 2024
概括
高血糖症通过改变内皮细胞分泌体,损害了血脑屏障 (BBB) 的完整性,这反过来影响了细胞周围细胞的细胞因子,突出了脑血管损伤中的内皮细胞-细胞周围细胞轴.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 血管生物学 血管生物学
背景情况:
- 血脑屏障 (BBB) 的破坏是缺血性中风后高血糖引起的脑血管损伤的关键因素.
- 由内皮细胞 (EC) 释放的血管活性剂可能会影响BBB功能障碍.
研究的目的:
- 研究高血糖症对内皮细胞特征及其分泌物的影响.
- 为了确定高血糖或正常血糖EC的分泌物如何影响危险细胞细胞因子的概况.
主要方法:
- 使用了人类BBB (大脑微血管EC,星细胞,周细胞) 的三重细胞培养模型.
- 过高血糖 (25mM D-葡萄糖) 和正常血糖 (5.5mM D-葡萄糖) 条件适用于72小时.
- 测量包括超内皮电阻,细胞流量,紧结蛋白分析和细胞因子分析.
主要成果:
- 高血糖会损害BBB的完整性和功能,通过降低电阻和增加的光素流量来表明.
- 观察到紧接口蛋白质溶解 (zonula occludens-1) 和应力纤维的形成.
- 高血糖EC分泌改变了细胞周细胞的细胞因子释放,增加了某些因素,减少了其他因素.
- 尽管分泌组合发生了变化,但在两种葡萄糖条件下,EC管原体容量仍然相似.
结论:
- 由高血糖引起的BBB干扰涉及复杂的机制.
- 内皮细胞-细胞轴在治疗高血糖脑血管损伤的治疗策略的开发中发挥着至关重要的作用.
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