波林缺乏或等离子体副本数量的增加介导着耐卡巴胺抗性大肠杆菌 (Escherichia coli) 耐药性的演变
Guoxiu Xiang1, Zhiwei Zhao1, Shebin Zhang2
1Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, People's Republic of China.
Emerging microbes & infections
|May 7, 2024
概括
细菌对梅洛等抗生素的耐药性通过两个主要途径进化:降低外膜蛋白的调节或增加等离子体拷贝数量. 这些机制在抗生素压力下增强了细菌的生存.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 抗生素耐药性是一个日益增长的全球健康威胁.
- 结合性等离子体在抗生素耐药性的传播中发挥着重要作用.
- 了解耐药性演变机制对于开发有效的治疗方法至关重要.
研究的目的:
- 研究细菌宿主和结合性等离子体中耐药性进化的机制.
- 为了确定不同度的美罗胺压力如何影响电阻.
- 识别与耐药性相关的特定遗传和转录性变化.
主要方法:
- 在美罗胺压力下,大肠杆菌菌株的实验室演变.
- 确定最小抑制度 (MIC) 和结合频率.
- 全基因组测序和转录分析.
- 重组实验用于验证宿主和等离子体因子.
主要成果:
- 两个克隆通过在低梅罗烯压力下下调外膜蛋白 *ompF* 表达来增加MIC.
- 所有克隆均表现出MIC和结合频率在较高的美罗胺压力下增加,这是由于等离子体拷贝数量的增加所致.
- 一个特定的*repA*突变 (D140Y) 与增加的等离子体拷贝数量有关.
结论:
- 细菌宿主和等离子体可以通过减少外膜蛋白表达或增加等离子体副本数量来发展耐药性.
- 这些适应性取决于抗微生物压力的度.
- 这项研究阐明了推动抗生素耐药性演变的关键分子机制.
相关概念视频
00:57Antibiotic Selection
53.2K
Overview
53.2K
00:46Genomic DNA in Prokaryotes
43.8K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
43.8K
02:46Genome Copying Errors
4.2K
DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their survival. Therefore, the copying errors are checked and repaired at three levels.
4.2K