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Updated: Jun 27, 2025

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没有中间净化的二次同热放大光生物传感器,用于超敏感检测循环瘤DNA

Zhaojie Wu1, Hongshan Zheng1, Yongjun Bian1

  • 1The Higher Educational Key Laboratory for Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, 422 Siming Nan Road, Xiamen 361005, China. sunliping@xmu.edu.cn.

The Analyst
|May 7, 2024
PubMed
概括

一种新的无净化生物传感器通过结合NEMA和CHA反应,能够超灵敏地检测循环瘤DNA (ctDNA). 这一进步为早期癌症诊断提供了一种方便且高度敏感的工具.

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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