单独的Streptococcus pyogenes调节器RofA表现出PRD含有毒性调节器的特征
Meaghan T Hart1, Joseph S Rom1, Yoann Le Breton1
1Department of Cell Biology and Molecular Genetics, Maryland Pathogen Research Institute, College Park, Maryland, USA.
Infection and immunity
|May 7, 2024
概括
群A链球菌毒性调节剂RofA被糖吸收系统化,将碳水化合物可用性与像皮利和囊这样的关键毒性因子的表达联系起来.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 病变的发生和发病.
背景情况:
- 杆菌 pyogenes (A组杆菌,GAS) 是一个适应营养可用性的人类病原体.
- 酸转移酶系统 (PTS) 调节碳水化合物吸收和代谢基因.
- 转录调节器上的PTS调节域 (PRD) 调节适应; GAS具有像RofA.这样的PRD含有毒性调节器 (PCVR).
研究的目的:
- 为了调查RofA,一个GAS毒性调节器,是否被PTS酸化.
- 为了确定RofA酸化是否将碳水化合物水平与病毒性基因表达联系起来.
- 为了确定RofA regulon及其对GAS毒性因子的影响.
主要方法:
- RNA测序以确定GAS菌株5448.8中的RofA规律子.
- 采用纯化的RofA和PTS蛋白 (EI,HPr) 的体外酸化试验.
- 在不同的葡萄糖条件下在体内检测化RofA,并分析RofA突变菌株.
主要成果:
- RofA调节了pilus操作子 (下调) 和囊操作子 (上调) 的表达.
- 通过PTS蛋白EI和HPr的RofA酸化在体外得到证实.
- 在低血糖条件下,酸化RofA在体内检测到,但在高血糖条件下没有.
- 假定PRD中的突变影响了RofA的酸化,这表明了额外的酸化位.
结论:
- RofA作为PCVR功能,将碳水化合物代谢与GAS中的毒性基因调节相结合.
- 通过PTS介导的RofA酸化可能协调了GAS适应营养环境的过程.
- 这种机制影响了关键的GAS毒性因子的表达,包括囊和 pili.
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